Histone H3K27me3 antibody (mAb) | Active Motif 61017 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K27me3 antibody (mAb)

catalog :

61017

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0323

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 22

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; loading ...; fig 4d western blot; mouse; 1:1000; loading ...; fig 4b western blot; human; 1:1000; loading ...; fig 3b	Keane L, Cheray M, Saidi D, Kirby C, Friess L, González Rodríguez P, et al. Inhibition of microglial EZH2 leads to anti-tumoral effects in pediatric diffuse midline gliomas. Neurooncol Adv. 2021;3:vdab096 pubmed publisher
immunocytochemistry; human; 1:250; loading ...; fig 1a	Farhy C, Hariharan S, Ylanko J, Orozco L, Zeng F, Pass I, et al. Improving drug discovery using image-based multiparametric analysis of the epigenetic landscape. elife. 2019;8: pubmed publisher
immunocytochemistry; mouse; loading ...; fig 2s1d, 3g	Del Rosario B, Kriz A, Del Rosario A, Anselmo A, Fry C, White F, et al. Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition. elife. 2019;8: pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig 1a	Inoue A, Chen Z, Yin Q, Zhang Y. Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells. Genes Dev. 2018;32:1525-1536 pubmed publisher
immunocytochemistry; mouse; fig 8	Brideau N, Coker H, Gendrel A, Siebert C, Bezstarosti K, Demmers J, et al. Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome. Mol Cell Biol. 2015;35:4053-68 pubmed publisher
chromatin immunoprecipitation; human western blot; human; loading ...; fig st2	Tiffen J, Gunatilake D, Gallagher S, Gowrishankar K, Heinemann A, Cullinane C, et al. Targeting activating mutations of EZH2 leads to potent cell growth inhibition in human melanoma by derepression of tumor suppressor genes. Oncotarget. 2015;6:27023-36 pubmed publisher
chromatin immunoprecipitation; human; fig 6	Liu X, Chen X, Rycaj K, Chao H, Deng Q, Jeter C, et al. Systematic dissection of phenotypic, functional, and tumorigenic heterogeneity of human prostate cancer cells. Oncotarget. 2015;6:23959-86 pubmed
immunohistochemistry; mouse; fig 2a	Yamada N, Hasegawa Y, Yue M, Hamada T, Nakagawa S, Ogawa Y. Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome. PLoS Genet. 2015;11:e1005430 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 1	Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher
	Chen Z, Djekidel M, Zhang Y. Distinct dynamics and functions of H2AK119ub1 and H3K27me3 in mouse preimplantation embryos. Nat Genet. 2021;53:551-563 pubmed publisher
	Kubo N, Ishii H, Xiong X, Bianco S, Meitinger F, Hu R, et al. Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation. Nat Struct Mol Biol. 2021;28:152-161 pubmed publisher
	Healy E, Mucha M, Glancy E, Fitzpatrick D, Conway E, Neikes H, et al. PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation. Mol Cell. 2019;76:437-452.e6 pubmed publisher
	Wang S, Zhang C, Hasson D, Desai A, SenBanerjee S, Magnani E, et al. Epigenetic Compensation Promotes Liver Regeneration. Dev Cell. 2019;: pubmed publisher
	Harutyunyan A, Krug B, Chen H, Papillon Cavanagh S, Zeinieh M, De Jay N, et al. H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. Nat Commun. 2019;10:1262 pubmed publisher
	Hiramuki Y, Tapscott S. Identification of SMCHD1 domains for nuclear localization, homo-dimerization, and protein cleavage. Skelet Muscle. 2018;8:24 pubmed publisher
	Du J, Kirk B, Zeng J, Ma J, Wang Q. Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes. Nucleic Acids Res. 2018;46:8848-8864 pubmed publisher
	Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, et al. A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Mol Cell. 2018;70:408-421.e8 pubmed publisher
	Yue M, Ogawa Y. CRISPR/Cas9-mediated modulation of splicing efficiency reveals short splicing isoform of Xist RNA is sufficient to induce X-chromosome inactivation. Nucleic Acids Res. 2018;46:e26 pubmed publisher
	Yue M, Ogawa A, Yamada N, Charles Richard J, Barski A, Ogawa Y. Xist RNA repeat E is essential for ASH2L recruitment to the inactive X and regulates histone modifications and escape gene expression. PLoS Genet. 2017;13:e1006890 pubmed publisher
	Pawlyn C, Bright M, Buros A, Stein C, Walters Z, Aronson L, et al. Overexpression of EZH2 in multiple myeloma is associated with poor prognosis and dysregulation of cell cycle control. Blood Cancer J. 2017;7:e549 pubmed publisher
	O Hara S, Splinter P, Trussoni C, Pisarello M, Loarca L, Splinter N, et al. ETS Proto-oncogene 1 Transcriptionally Up-regulates the Cholangiocyte Senescence-associated Protein Cyclin-dependent Kinase Inhibitor 2A. J Biol Chem. 2017;292:4833-4846 pubmed publisher
	Kelsey A, Yang C, Leung D, Minks J, Dixon McDougall T, Baldry S, et al. Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST. Genome Biol. 2015;16:208 pubmed publisher

product information

Catalog Number :

61017

Product Name :

Histone H3K27me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0323

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.

Uses :

ChIP, DB, TR-FRET, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The creation of this antibody was described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.This antibody has been used in the following references: ChIP: Ohhata T et al. (2008) Development 135: 227-235; Luco RF et al. (2010) Cell 327: 996-1000.IF: Hayashi-Takanaka Y et al. (2011) Nucl Acid Res 39: 6475-6488.WB: Hayashi-Takanaka Y et al. (2011) Nucl Acid Res 39: 6475-6488.