Histone H3K27me1 antibody (mAb) | Active Motif 61015 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K27me1 antibody (mAb)

catalog :

61015

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0321

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 5

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse; loading ...; fig 5a western blot; mouse; 1:1000; loading ...; fig 1b	Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1d	Lima Fernandes E, Murison A, da Silva Medina T, Wang Y, Ma A, Leung C, et al. Targeting bivalency de-represses Indian Hedgehog and inhibits self-renewal of colorectal cancer-initiating cells. Nat Commun. 2019;10:1436 pubmed publisher
western blot; mouse; fig s6d	Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher
western blot; human; fig s9e	Ntziachristos P, Tsirigos A, Welstead G, Trimarchi T, Bakogianni S, Xu L, et al. Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic leukaemia. Nature. 2014;514:513-7 pubmed publisher
	Healy E, Mucha M, Glancy E, Fitzpatrick D, Conway E, Neikes H, et al. PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation. Mol Cell. 2019;76:437-452.e6 pubmed publisher

product information

Catalog Number :

61015

Product Name :

Histone H3K27me1 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0321

Isotype :

IgG2a

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 monomethyl Lys27 antibody was raised against a peptide containing monomethyl Lys27 of human Histone H3.

Uses :

ChIP, DB, TR-FRET, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: The creation of this antibody was described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.