5-Hydroxymethylcytosine (5-hmC) antibody (mAb) | Active Motif 39999 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

5-Hydroxymethylcytosine (5-hmC) antibody (mAb)

catalog :

39999

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

59.1

reactivity :

mouse, pigs , Xenopus laevis

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; pigs ; 2 ug/time	Jia Y, Li R, Cong R, Yang X, Sun Q, Parvizi N, et al. Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter. PLoS ONE. 2013;8:e63855 pubmed publisher
immunohistochemistry; mouse; 1:500	Li Y, O Neill C. 5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote. PLoS ONE. 2013;8:e63689 pubmed publisher
immunoprecipitation; pigs ; 1 ug	Li R, Zou H, Jia Y, Zhao R. Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets. BMC Vet Res. 2013;9:87 pubmed publisher
immunocytochemistry; Xenopus laevis; 1:4000	Morgan G, Jones P, Bellini M. Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin. Chromosome Res. 2012;20:925-42 pubmed publisher

product information

Catalog Number :

39999

Product Name :

5-Hydroxymethylcytosine (5-hmC) antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

59.1

Isotype :

IgG2a

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

In addition to this monoclonal, Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal (39791). The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 ul per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well.DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so.It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.

Immunogen :

This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Uses :

DB, IP, MeDIP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.