Ago1/2/3 antibody (mAb) | Active Motif 39937 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Ago1/2/3 antibody (mAb)

catalog :

39937

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A8

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

product information

Catalog Number :

39937

Product Name :

Ago1/2/3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

2A8

Isotype :

IgG

CrossReactivity :

Human

Background :

The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.

Immunogen :

This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-859 of human Ago2.

Uses :

ChIP, IF, IHC, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following publications: ChIP: Schwartz JC et al. (2009) Nat Struct Molec Biol 15: 842-848.IF: Nelson PT et al. (2007) RNA 13: 1787-1792.IHC: Nelson PT et al. (2007) RNA 13: 1787-1792.IP: Nelson PT et al. (2007) RNA 13: 1787-1792.HITS-CLIP: Chi SW et al. (2009) Nature 460: 479-486.RIP-ChIP: Schwartz JC et al. (2009) Nat Struct Molec Biol 15: 842-848.WB: Nelson PT et al. (2007) RNA 13: 1787-1792.