Histone H3K4me2 antibody (pAb) | Active Motif 39913 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K4me2 antibody (pAb)

catalog :

39913

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, brewer's yeast

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; 1:5000; loading ...; fig 1a	Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher

product information

Catalog Number :

39913

Product Name :

Histone H3K4me2 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

100 ug

Isotype :

IgG

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Immunogen :

This Histone H3 dimethyl Lys4 antibody was raised against a peptide including dimethyl-lysine 4 of histone H3.

Uses :

ChIP, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.