EZH2 antibody (mAb) | Active Motif 39875 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

EZH2 antibody (mAb)

catalog :

39875

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC22

The same clone is also sold as:

Cell Signaling Technology: 3147
Invitrogen: 50-9867-82, 14-9867-37, 14-9867-80, 14-9867-82

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq, RNA immunoprecipitation

citations: 15

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig s10b western blot; human; 1:10,000; fig 1b	Zhang Q, Agius S, Flanigan S, Uckelmann M, Levina V, Owen B, et al. PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis. Nat Commun. 2021;12:4592 pubmed publisher
western blot; mouse; loading ...; fig 4e	Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
RNA immunoprecipitation; human; fig 6 chromatin immunoprecipitation; human; fig 6	Ranzani V, Rossetti G, Panzeri I, Arrigoni A, Bonnal R, Curti S, et al. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Nat Immunol. 2015;16:318-325 pubmed publisher
ChIP-Seq; human chromatin immunoprecipitation; human	Englert N, Luo G, Goldstein J, Surapureddi S. Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. J Biol Chem. 2015;290:2264-78 pubmed publisher
chromatin immunoprecipitation; mouse	Wang A, Pan D, Lee Y, Martinez G, Feng X, Dong C. Cutting edge: Smad2 and Smad4 regulate TGF-?-mediated Il9 gene expression via EZH2 displacement. J Immunol. 2013;191:4908-12 pubmed publisher
chromatin immunoprecipitation; human	Bengani H, Mendiratta S, Maini J, Vasanthi D, Sultana H, Ghasemi M, et al. Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome. PLoS ONE. 2013;8:e67217 pubmed publisher
immunoprecipitation; human; 5 ul	Rossetto C, Tarrant Elorza M, Pari G. Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells. PLoS Pathog. 2013;9:e1003366 pubmed publisher
western blot; human	Lin S, Shen H, Li J, Tang S, Gu Y, Chen Z, et al. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem. 2013;288:6238-47 pubmed publisher
chromatin immunoprecipitation; human western blot; human	Murata T, Kondo Y, Sugimoto A, Kawashima D, Saito S, Isomura H, et al. Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells. J Virol. 2012;86:4752-61 pubmed publisher
	Huang S, Fantini D, Merrill B, Bagchi S, Guzman G, Raychaudhuri P. DDB2 Is a Novel Regulator of Wnt Signaling in Colon Cancer. Cancer Res. 2017;77:6562-6575 pubmed publisher
	Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
	Lawrence C, Baldwin A. Non-Canonical EZH2 Transcriptionally Activates RelB in Triple Negative Breast Cancer. PLoS ONE. 2016;11:e0165005 pubmed publisher
	Akizu N, García M, Estarás C, Fueyo R, Badosa C, de la Cruz X, et al. EZH2 regulates neuroepithelium structure and neuroblast proliferation by repressing p21. Open Biol. 2016;6:150227 pubmed publisher
	Mitić T, Caporali A, Floris I, Meloni M, Marchetti M, Urrutia R, et al. EZH2 modulates angiogenesis in vitro and in a mouse model of limb ischemia. Mol Ther. 2015;23:32-42 pubmed publisher
	Woellmer A, Arteaga Salas J, Hammerschmidt W. BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression. PLoS Pathog. 2012;8:e1002902 pubmed publisher

product information

Catalog Number :

39875

Product Name :

EZH2 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

AC22

Isotype :

IgG2a

CrossReactivity :

Human, Mouse

Background :

EZH2 M-bM-^@M-^S (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Immunogen :

This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

Uses :

ChIP, IF, IP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references: ChIP: Bracken AP et al. (2007) Genes Dev 21: 535-540; Pasini D et al. (2007) Molec Cell Biol 27: 3769-3779; Pasini D et al. (2008) Genes Dev 22: 1345-1355.IP: Pasini D et al. (2008) Genes Dev 22: 1345-1355.