5-Hydroxymethylcytosine (5-hmC) antibody (pAb) | Active Motif 39791 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

catalog :

39791

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, dot blot

citations: 10

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; loading ...; fig 4a	Imai Y, Biot M, Clément J, Teragaki M, Urbach S, Robert T, et al. PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment. elife. 2020;9: pubmed publisher
western blot; mouse; 1:1000; loading ...; fig e7f	DiTroia S, Percharde M, Guerquin M, Wall E, Collignon E, Ebata K, et al. Maternal vitamin C regulates reprogramming of DNA methylation and germline development. Nature. 2019;573:271-275 pubmed publisher
immunohistochemistry - paraffin section; pigs ; 1:1500; loading ...; fig 1g	Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig 2h	Yamagata K, Kobayashi A. The cysteine-rich domain of TET2 binds preferentially to mono- and dimethylated histone H3K36. J Biochem. 2017;161:327-330 pubmed publisher
immunoprecipitation; domestic rabbit; 1:100; fig 's6'	Suzuki A, Hirasaki M, Hishida T, Wu J, Okamura D, Ueda A, et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun. 2016;7:11056 pubmed publisher
immunohistochemistry; mouse; 1:500	Murakami K, GÃ¼nesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher
	Papin C, Ibrahim A, Gras S, Velt A, Stoll I, Jost B, et al. Combinatorial DNA methylation codes at repetitive elements. Genome Res. 2017;27:934-946 pubmed publisher
	Lo Nigro A, de Jaime Soguero A, Khoueiry R, Cho D, Ferlazzo G, Perini I, et al. PDGFR?+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors. Stem Cell Reports. 2017;8:318-333 pubmed publisher
	Thienpont B, Steinbacher J, Zhao H, D Anna F, Kuchnio A, Ploumakis A, et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. 2016;537:63-68 pubmed publisher
	Heyward F, Gilliam D, Coleman M, Gavin C, Wang J, Kaas G, et al. Obesity Weighs down Memory through a Mechanism Involving the Neuroepigenetic Dysregulation of Sirt1. J Neurosci. 2016;36:1324-35 pubmed publisher

product information

Catalog Number :

39791

Product Name :

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

100 ug

Isotype :

IgG

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 ul per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well.DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so.It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.

Immunogen :

This 5-Hydroxymethylcytidine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Uses :

DB, IP, MeDIP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references:MeDIP: Stroud H et al. (2011) Genome Biol 12: R54.