5-Hydroxymethylcytosine (5-hmC) antibody (pAb) | Active Motif 39769 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

catalog :

39769

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, dot blot, other

citations: 25

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 1b	Ostriker A, Xie Y, Chakraborty R, Sizer A, Bai Y, Ding M, et al. TET2 Protects Against Vascular Smooth Muscle Cell Apoptosis and Intimal Thickening in Transplant Vasculopathy. Circulation. 2021;144:455-470 pubmed publisher
immunohistochemistry - paraffin section; human immunohistochemistry; human	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
	Zhang M, Wang J, Zhang K, Lu G, Liu Y, Ren K, et al. Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. Nat Commun. 2021;12:5091 pubmed publisher
	Moyon S, Frawley R, Maréchal D, Huang D, Marshall Phelps K, Kegel L, et al. TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice. Nat Commun. 2021;12:3359 pubmed publisher
	Huang G, Liu L, Wang H, Gou M, Gong P, Tian C, et al. Tet1 Deficiency Leads to Premature Reproductive Aging by Reducing Spermatogonia Stem Cells and Germ Cell Differentiation. iScience. 2020;23:100908 pubmed publisher
	Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, et al. UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I. Cell Death Dis. 2020;11:142 pubmed publisher
	Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
	Morris J, Yashinskie J, Koche R, Chandwani R, Tian S, Chen C, et al. α-Ketoglutarate links p53 to cell fate during tumour suppression. Nature. 2019;573:595-599 pubmed publisher
	Zhang J, Tan P, Guo L, Gong J, Ma J, Li J, et al. p53-dependent autophagic degradation of TET2 modulates cancer therapeutic resistance. Oncogene. 2019;38:1905-1919 pubmed publisher
	Zhang P, Rausch C, Hastert F, Boneva B, Filatova A, Patil S, et al. Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res. 2017;45:7118-7136 pubmed publisher
	Ma S, Wan X, Deng Z, Shi L, Hao C, Zhou Z, et al. Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs. J Exp Med. 2017;214:1471-1491 pubmed publisher
	Liu Y, Zhang B, Meng X, Korn M, Parent J, Lu L, et al. UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures. Epigenetics. 2017;12:551-560 pubmed publisher
	Zhong X, Wang Q, Li J, Zhang Y, An X, Hou J. Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro. Sci Rep. 2017;7:43539 pubmed publisher
	Jaiswal M, Bhar S, Vemula H, Prakash S, Ponnaluri V, Gutheil W, et al. Convenient expression, purification and quantitative liquid chromatography-tandem mass spectrometry-based analysis of TET2 5-methylcytosine demethylase. Protein Expr Purif. 2017;132:143-151 pubmed publisher
	Lemonnier F, Cairns R, Inoue S, Li W, Dupuy A, Broutin S, et al. The IDH2 R172K mutation associated with angioimmunoblastic T-cell lymphoma produces 2HG in T cells and impacts lymphoid development. Proc Natl Acad Sci U S A. 2016;113:15084-15089 pubmed publisher
	Shu L, Sun W, Li L, Xu Z, Lin L, Xie P, et al. Genome-wide alteration of 5-hydroxymenthylcytosine in a mouse model of Alzheimer's disease. BMC Genomics. 2016;17:381 pubmed publisher
	Yu P, Ji L, Lee K, Yu M, He C, Ambati S, et al. Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine. PLoS ONE. 2016;11:e0154949 pubmed publisher
	Hohos N, Lee K, Ji L, Yu M, Kandasamy M, Phillips B, et al. DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes. J Immunol Methods. 2016;436:1-15 pubmed publisher
	Petrussa L, Van De Velde H, De Rycke M. Similar kinetics for 5-methylcytosine and 5-hydroxymethylcytosine during human preimplantation development in vitro. Mol Reprod Dev. 2016;83:594-605 pubmed publisher
	Gao J, Ma Y, Fu H, Luo Q, Wang Z, Xiao Y, et al. Non-catalytic roles for TET1 protein negatively regulating neuronal differentiation through srGAP3 in neuroblastoma cells. Protein Cell. 2016;7:351-61 pubmed publisher
	Shi X, Yu Y, Luo M, Zhang Z, Shi S, Feng X, et al. Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma. PLoS ONE. 2016;11:e0153100 pubmed publisher
	Weber A, Krawczyk C, Robertson A, KuÅ›nierczyk A, VÃ¥gbÃ¸ C, Schuermann D, et al. Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism. Nat Commun. 2016;7:10806 pubmed publisher
	Brasa S, Mueller A, Jacquemont S, Hahne F, Rozenberg I, Peters T, et al. Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome. Clin Epigenetics. 2016;8:15 pubmed publisher
	Munari E, Chaux A, Vaghasia A, Taheri D, Karram S, Bezerra S, et al. Global 5-Hydroxymethylcytosine Levels Are Profoundly Reduced in Multiple Genitourinary Malignancies. PLoS ONE. 2016;11:e0146302 pubmed publisher
	Li Y, Seah M, O NEILL C. Mapping global changes in nuclear cytosine base modifications in the early mouse embryo. Reproduction. 2016;151:83-95 pubmed publisher

product information

Catalog Number :

39769

Product Name :

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

100 ul

Isotype :

Serum

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 ul per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010);the purified IgG version (39791) is likely to work in IF as well.DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.

Immunogen :

This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Uses :

DB, IF, IHC, IP, MeDIP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used as described below: DB: Ito S. et al. (2010) Nature 466: 1129-1133, Szulwach KE et al. (2011) Nature Neurosci doi:10.1038/nn.2959; Nestor CE. et al. (2011) Genome Res ePub. IHC: MM-CM-<nzel M. et al. (2010) Angew Chem Int Ed Engl 49: 5375 - 5377; Haffner MC. et al. (2011) Oncotarget 2: 627-637, Szulwach KE et al. (2011) Nature Neurosci doi:10.1038/nn.2959. IF: Ito S. et al. (2010) Nature 466: 1129-1133. MeDIP: Nestor CE. et al. (2011) Genome Res ePub. MeDIP-Seq: Stroud et al. (2011) Genome Biology 12:R54.