Histone H3 antibody (mAb) | Active Motif 39763 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3 antibody (mAb)

catalog :

39763

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0301

reactivity :

human, mouse, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:100; loading ...; fig 2a	Kuhn T, Pascual García P, Gozalo A, Little S, Capelson M. Chromatin targeting of nuclear pore proteins induces chromatin decondensation. J Cell Biol. 2019;218:2945-2961 pubmed publisher
western blot; human; loading ...; fig 5b	Perez Leal O, Barrero C, Merali S. Pharmacological stimulation of nuclear factor (erythroid-derived 2)-like 2 translation activates antioxidant responses. J Biol Chem. 2017;292:14108-14121 pubmed publisher
western blot; human; loading ...; fig 2b	Pal S, Graves H, Ohsawa R, Huang T, Wang P, Harmacek L, et al. The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells. PLoS ONE. 2016;11:e0155409 pubmed publisher
immunoprecipitation; human; fig 1 western blot; human; fig 1	Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
western blot; human; fig 1	Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 1b	Fukuda A, Mitani A, Miyashita T, Umezawa A, Akutsu H. Chromatin condensation of Xist genomic loci during oogenesis in mice. Development. 2015;142:4049-55 pubmed publisher
proximity ligation assay; human; 1:4000; fig 2 immunocytochemistry; human; 1:4000; fig 2	Zhan Y, Kost Alimova M, Shi X, Leo E, Bardenhagen J, Shepard H, et al. Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery. Epigenetics Chromatin. 2015;8:37 pubmed publisher
western blot; human; loading ...; fig 1b	Wagner W, Ciszewski W, Kania K. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal. 2015;13:36 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 4f	Chen H, Huang W, Yang L, Lin C. The PTEN-AKT-mTOR/RICTOR Pathway in Nasal Natural Killer Cell Lymphoma Is Activated by miR-494-3p via PTEN But Inhibited by miR-142-3p via RICTOR. Am J Pathol. 2015;185:1487-99 pubmed publisher
ELISA; human	Pacaud R, Cheray M, Nadaradjane A, Vallette F, Cartron P. Histone H3 phosphorylation in GBM: a new rational to guide the use of kinase inhibitors in anti-GBM therapy. Theranostics. 2015;5:12-22 pubmed publisher
immunohistochemistry; mouse; loading ...; fig s2 western blot; mouse; loading ...; fig 3c	Arnandis T, Ferrer Vicens I, Torres L, García C, García Trevijano E, Zaragoza R, et al. Differential functions of calpain 1 during epithelial cell death and adipocyte differentiation in mammary gland involution. Biochem J. 2014;459:355-68 pubmed publisher
chromatin immunoprecipitation; mouse	Ohmori S, Takai J, Ishijima Y, Suzuki M, Moriguchi T, Philipsen S, et al. Regulation of GATA factor expression is distinct between erythroid and mast cell lineages. Mol Cell Biol. 2012;32:4742-55 pubmed publisher
immunocytochemistry; human; 1:200	Cerf A, Tian H, Craighead H. Ordered arrays of native chromatin molecules for high-resolution imaging and analysis. ACS Nano. 2012;6:7928-34 pubmed
immunocytochemistry; human immunocytochemistry; mouse	De Cecco M, Jeyapalan J, Zhao X, Tamamori Adachi M, Sedivy J. Nuclear protein accumulation in cellular senescence and organismal aging revealed with a novel single-cell resolution fluorescence microscopy assay. Aging (Albany NY). 2011;3:955-67 pubmed
	Li J, Dong A, Saydaminova K, Chang H, Wang G, Ochiai H, et al. Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells. Cell. 2019;: pubmed publisher
	Garcia Moreno S, Futtner C, Salamone I, Gonen N, Lovell Badge R, Maatouk D. Gonadal supporting cells acquire sex-specific chromatin landscapes during mammalian sex determination. Dev Biol. 2019;446:168-179 pubmed publisher
	El Daher M, Cagnard N, Gil M, Da Cruz M, Leveau C, Sepulveda F, et al. Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure. Cell Discov. 2018;4:61 pubmed publisher
	Cheng C, Qi Y, Wang Y, Chi K, Chung Y, Ouyang C, et al. Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction. Commun Biol. 2018;1:178 pubmed publisher
	Valsecchi C, Basilicata M, Semplicio G, Georgiev P, Gutierrez N, Akhtar A. Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells. Nat Commun. 2018;9:3626 pubmed publisher
	Fujita T, Kitaura F, Yuno M, Suzuki Y, Sugano S, Fujii H. Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line. DNA Res. 2017;24:537-548 pubmed publisher
	Ohhata T, Matsumoto M, Leeb M, Shibata S, Sakai S, Kitagawa K, et al. Histone H3 Lysine 36 Trimethylation Is Established over the Xist Promoter by Antisense Tsix Transcription and Contributes to Repressing Xist Expression. Mol Cell Biol. 2015;35:3909-20 pubmed publisher
	van de Werken C, van der Heijden G, Eleveld C, Teeuwssen M, Albert M, Baarends W, et al. Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications. Nat Commun. 2014;5:5868 pubmed publisher
	Murphy P, Cipriany B, Wallin C, Ju C, Szeto K, Hagarman J, et al. Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells. Proc Natl Acad Sci U S A. 2013;110:7772-7 pubmed publisher
	Jones J, Bhattacharyya A, Simkus C, Vallieres B, Veenstra T, Zhou M. The RAG1 V(D)J recombinase/ubiquitin ligase promotes ubiquitylation of acetylated, phosphorylated histone 3.3. Immunol Lett. 2011;136:156-62 pubmed publisher

product information

Catalog Number :

39763

Product Name :

Histone H3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

MABI 0301

Isotype :

IgG2b

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Immunogen :

This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.

Uses :

ChIP, TR-FRET, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used in ChIP assays as described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.