BubR1 antibody (mAb) | Active Motif 39747 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

BubR1 antibody (mAb)

catalog :

39747

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8G1

The same clone is also sold as:

Santa Cruz Biotechnology: sc-47744
Invitrogen: MA1-16577, MA5-16037, MA5-16035, MA5-16036, MA5-16038, MA5-16039
OriGene: TA301741
Novus Biologicals: NBP2-80606, NB100-353
Rockland Immunochemicals: 200-301-902
Abnova: MAB1929

reactivity :

human, mouse

application :

western blot, immunocytochemistry

product information

Catalog Number :

39747

Product Name :

BubR1 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

8G1

Isotype :

IgG

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

BubR1 (also known as BUB1B, MAD3L and SSK1) is an essential component of the mitotic spindle integrity checkpoint and is required for the normal progression of mitosis, and thus can act as a tumor suppressor. It encodes a serine / threonine kinase localized to the kinetochore and is involved in spindle checkpoint function. BubR1 plays a role in the inhibition of the anaphase-promoting complex (APC), delaying the onset of anaphase and ensuring proper chromosome segregation. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle and the chromosomes are aligned on the metaphase plate. Another function involves the monitoring of kinetochore activities that depend on the kinetochore motor CENP-E. It is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. BubR1 is autophosphorylated, and BubR1 kinase activity is stimulated by association with the centromere protein CENP-E. Phosphorylation at Ser670 and Ser1043 occurs at kinetochores upon mitotic entry with dephosphorylation occurring at the onset of anaphase. Impaired spindle checkpoint function has been found in many forms of cancer. Defects in BubR1 are involved in mosaic variegated aneuploidy syndrome (MVA) and premature chromatid separation trait (PCS). BubR1 interacts with CENP-E, CENP-F, mitosin, PLK1, CASC5, BUB3 and the anaphase-promoting complex (APC). It is part of a complex containing BUB3 and CDC2.

Immunogen :

Recombinant protein corresponding to amino acids 1-350 of human BubR1.

Uses :

IF, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody is described in Liu, S.T. et al (2003) Nat Cell Biol 5: 341M-bM-^@M-^S345.