Histone H3K27ac antibody (mAb) | Active Motif 39685 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K27ac antibody (mAb)

catalog :

39685

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0309

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq, other

citations: 19

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 5c	Huang H, Hu J, Maryam A, Huang Q, Zhang Y, Ramakrishnan S, et al. Defining super-enhancer landscape in triple-negative breast cancer by multiomic profiling. Nat Commun. 2021;12:2242 pubmed publisher
chromatin immunoprecipitation; human; fig 4b	Wu S, Turner K, Nguyen N, Raviram R, Erb M, Santini J, et al. Circular ecDNA promotes accessible chromatin and high oncogene expression. Nature. 2019;575:699-703 pubmed publisher
immunocytochemistry; human; 1:250; loading ...; fig 1a	Farhy C, Hariharan S, Ylanko J, Orozco L, Zeng F, Pass I, et al. Improving drug discovery using image-based multiparametric analysis of the epigenetic landscape. elife. 2019;8: pubmed publisher
other; human; loading ...; fig s7k	Li J, Dong A, Saydaminova K, Chang H, Wang G, Ochiai H, et al. Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells. Cell. 2019;: pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 4a	Żylicz J, Bousard A, Zumer K, Dossin F, Mohammad E, da Rocha S, et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell. 2019;176:182-197.e23 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 7d	Haldeman J, Conway A, Arlotto M, Slentz D, Muoio D, Becker T, et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2019;47:e23 pubmed publisher
chromatin immunoprecipitation; mouse; fig 8d	Raices M, Bukata L, Sakuma S, Borlido J, Hernandez L, Hart D, et al. Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex. Dev Cell. 2017;41:540-554.e7 pubmed publisher
ChIP-Seq; mouse; loading ...; tbl 2 ChIP-Seq; human; loading ...; tbl 2	Busby M, Xue C, Li C, Farjoun Y, Gienger E, Yofe I, et al. Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. Epigenetics Chromatin. 2016;9:49 pubmed
chromatin immunoprecipitation; human; fig 4	Mendoza Parra M, Saravaki V, Cholley P, Blum M, Billoré B, Gronemeyer H. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification. F1000Res. 2016;5:54 pubmed publisher
chromatin immunoprecipitation; human; fig 1	Dheekollu J, Wiedmer A, Sentana Lledo D, Cassel J, Messick T, Lieberman P. HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus. J Virol. 2016;90:5353-5367 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 6b,6c	Kotomura N, Harada N, Ishihara S. The Proportion of Chromatin Graded between Closed and Open States Determines the Level of Transcripts Derived from Distinct Promoters in the CYP19 Gene. PLoS ONE. 2015;10:e0128282 pubmed publisher
	Hu J, Lai Y, Huang H, Ramakrishnan S, Pan Y, Ma V, et al. TCOF1 upregulation in triple-negative breast cancer promotes stemness and tumour growth and correlates with poor prognosis. Br J Cancer. 2022;126:57-71 pubmed publisher
	Davies A, Nouruzi S, Ganguli D, Namekawa T, Thaper D, Linder S, et al. An androgen receptor switch underlies lineage infidelity in treatment-resistant prostate cancer. Nat Cell Biol. 2021;23:1023-1034 pubmed publisher
	Martire S, Nguyen J, Sundaresan A, Banaszynski L. Differential contribution of p300 and CBP to regulatory element acetylation in mESCs. BMC Mol Cell Biol. 2020;21:55 pubmed publisher
	Dubois V, Gheeraert C, Vankrunkelsven W, Dubois Chevalier J, Dehondt H, Bobowski Gérard M, et al. Endoplasmic reticulum stress actively suppresses hepatic molecular identity in damaged liver. Mol Syst Biol. 2020;16:e9156 pubmed publisher
	Han M, Wei G, McManus C, Hillier L, Reinke V. Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression. BMC Genomics. 2019;20:500 pubmed publisher
	El Daher M, Cagnard N, Gil M, Da Cruz M, Leveau C, Sepulveda F, et al. Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure. Cell Discov. 2018;4:61 pubmed publisher
	Jia D, Augert A, Kim D, Eastwood E, Wu N, Ibrahim A, et al. Crebbp Loss Drives Small Cell Lung Cancer and Increases Sensitivity to HDAC Inhibition. Cancer Discov. 2018;8:1422-1437 pubmed publisher
	Gong H, Qian H, Ertl R, Astle C, Wang G, Harrison D, et al. Histone modifications change with age, dietary restriction and rapamycin treatment in mouse brain. Oncotarget. 2015;6:15882-90 pubmed

product information

Catalog Number :

39685

Product Name :

Histone H3K27ac antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Acetylated

Size :

100 ug

Clone :

MABI 0309

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation.Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 acetyl Lys27 antibody was raised against a synthetic peptide including acetyl-lysine 27 of human histone H3.

Uses :

ChIP, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used in ChIP assays as described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.