Histone H3K9me2 antibody (mAb) | Active Motif 39683 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K9me2 antibody (mAb)

catalog :

39683

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MABI 0307

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Watson M, Hedley D. Whole blood measurement of histone modifications linked to the epigenetic regulation of gene expression. Curr Protoc Cytom. 2015;71:6.36.1-9 pubmed publisher
chromatin immunoprecipitation; human western blot; human	Gregory B, Cheung V. Natural variation in the histone demethylase, KDM4C, influences expression levels of specific genes including those that affect cell growth. Genome Res. 2014;24:52-63 pubmed publisher
	Mutlu B, Chen H, Moresco J, Orelo B, Yang B, Gaspar J, et al. Regulated nuclear accumulation of a histone methyltransferase times the onset of heterochromatin formation in C. elegans embryos. Sci Adv. 2018;4:eaat6224 pubmed publisher

product information

Catalog Number :

39683

Product Name :

Histone H3K9me2 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

MABI 0307

Isotype :

IgG1

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 dimethyl Lys9 antibody was raised against a synthetic peptide containing dimethyl Lys9 of human histone H3.

Uses :

ChIP, DB, TR-FRET, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used in ChIP assays as described in Kimura, H. et al. (2008) Cell Struct Func 33: 61-73.