Histone H4K20me3 antibody (mAb) | Active Motif 39671 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H4K20me3 antibody (mAb)

catalog :

39671

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6F8-D9

The same clone is also sold as:

Invitrogen: MA5-18074
ImmuQuest: IQ558

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse	Takei Y, Yun J, Zheng S, Ollikainen N, Pierson N, White J, et al. Integrated spatial genomics reveals global architecture of single nuclei. Nature. 2021;590:344-350 pubmed publisher
western blot; mouse; 1:500; loading ...; fig 6h	Pedrotti S, Caccia R, Neguembor M, Garcia Manteiga J, Ferri G, De Palma C, et al. The Suv420h histone methyltransferases regulate PPAR-γ and energy expenditure in response to environmental stimuli. Sci Adv. 2019;5:eaav1472 pubmed publisher
western blot; human; fig 6	Nelson D, Jaber Hijazi F, Cole J, Robertson N, Pawlikowski J, Norris K, et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 2016;17:158 pubmed publisher
immunocytochemistry; mouse; 1:1000; fig 4,5,s5	Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama E, et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 2015;112:14641-6 pubmed publisher
	Brejc K, Bian Q, Uzawa S, Wheeler B, Anderson E, King D, et al. Dynamic Control of X Chromosome Conformation and Repression by a Histone H4K20 Demethylase. Cell. 2017;171:85-102.e23 pubmed publisher

product information

Catalog Number :

39671

Product Name :

Histone H4K20me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

100 ug

Clone :

6F8-D9

Isotype :

IgG

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Immunogen :

This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.

Uses :

ChIP, DB, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.