Ring1B antibody (mAb) | Active Motif 39663 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Ring1B antibody (mAb)

catalog :

39663

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; loading ...; tbl s3	Lau M, Schwartz M, Kundu S, Savol A, Wang P, Marr S, et al. Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning. Science. 2017;355:1081-1084 pubmed publisher
chromatin immunoprecipitation; mouse; fig s14a western blot; mouse; fig s15c	Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher
western blot; human	Murata K, Sato S, Haruta M, Goshima T, Chiba Y, Takahashi S, et al. Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochem Biophys Res Commun. 2015;458:470-475 pubmed publisher
chromatin immunoprecipitation; mouse	Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77 pubmed publisher
chromatin immunoprecipitation; human	Basu A, Wilkinson F, Colavita K, Fennelly C, Atchison M. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids Res. 2014;42:2208-23 pubmed publisher
western blot; human	Lin S, Shen H, Li J, Tang S, Gu Y, Chen Z, et al. Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program. J Biol Chem. 2013;288:6238-47 pubmed publisher
	Shukla S, Ying W, Gray F, Yao Y, Simes M, Zhao Q, et al. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain. Nat Chem Biol. 2021;17:784-793 pubmed publisher
	Harutyunyan A, Krug B, Chen H, Papillon Cavanagh S, Zeinieh M, De Jay N, et al. H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. Nat Commun. 2019;10:1262 pubmed publisher

product information

Catalog Number :

39663

Product Name :

Ring1B antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Isotype :

IgG

CrossReactivity :

Human, Monkey, Mouse

Background :

Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

Immunogen :

This Ring1B antibody was raised against the recombinant full-length mouse protein.

Uses :

ChIP, ChIP-chip, ChIP-Seq, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: 1. Atsuta, T. et. al. (2001) Hybridoma 20: 43-46.2. Suzuki, M. et. al. (2002) Development 129: 4171-4183.3. Fujimura, Y. et. al. (2006) Development 133: 2371-2381.4. Isono, K. et. al. (2005) Mol. Cell. Bio. 25: 6694-6706.