5-Methylcytosine (5-mC) antibody (mAb) | Active Motif 39649 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

5-Methylcytosine (5-mC) antibody (mAb)

catalog :

39649

quantity :

50 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33D3

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, dot blot

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig s4b	Deng X, Iwagawa T, Fukushima M, Suzuki Y, Watanabe S. Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1. Invest Ophthalmol Vis Sci. 2021;62:1 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 7c	Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, et al. UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I. Cell Death Dis. 2020;11:142 pubmed publisher
immunohistochemistry; mouse; 1:250; loading ...; fig s1, s4	Gulmez Karaca K, Kupke J, Brito D, Zeuch B, Thome C, Weichenhan D, et al. Neuronal ensemble-specific DNA methylation strengthens engram stability. Nat Commun. 2020;11:639 pubmed publisher
immunocytochemistry; human; 1:500; fig 1	Harrison J, Cornett E, Goldfarb D, DaRosa P, Li Z, Yan F, et al. Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1. elife. 2016;5: pubmed publisher
immunocytochemistry; mouse; 1:100; fig s1	McPherson N, Fullston T, Kang W, Sandeman L, Corbett M, Owens J, et al. Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers. Sci Rep. 2016;6:27010 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 3b	Borghesan M, Fusilli C, Rappa F, Panebianco C, Rizzo G, Oben J, et al. DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression. Cancer Res. 2016;76:594-606 pubmed publisher
immunohistochemistry - frozen section; human; 1:2000	Condliffe D, Wong A, Troakes C, Proitsi P, Patel Y, Chouliaras L, et al. Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain. Neurobiol Aging. 2014;35:1850-4 pubmed publisher
immunocytochemistry; mouse	Zhao X, Dai J, Ma Y, Mi Y, Cui D, Ju G, et al. Dynamics of ten-eleven translocation hydroxylase family proteins and 5-hydroxymethylcytosine in oligodendrocyte differentiation. Glia. 2014;62:914-26 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Jäwert F, Hassèus B, Kjeller G, Magnusson B, Sand L, Larsson L. Loss of 5-hydroxymethylcytosine and TET2 in oral squamous cell carcinoma. Anticancer Res. 2013;33:4325-8 pubmed
immunoprecipitation; mouse immunocytochemistry; mouse	Colquitt B, Allen W, Barnea G, Lomvardas S. Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity. Proc Natl Acad Sci U S A. 2013;110:14682-7 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000	Gambichler T, Sand M, Skrygan M. Loss of 5-hydroxymethylcytosine and ten-eleven translocation 2 protein expression in malignant melanoma. Melanoma Res. 2013;23:218-20 pubmed publisher
immunocytochemistry; Xenopus laevis; 1:200	Morgan G, Jones P, Bellini M. Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin. Chromosome Res. 2012;20:925-42 pubmed publisher
western blot; mouse	Xu X, Wang Q, Long Y, Zhang R, Wei X, Xing M, et al. Stress-mediated p38 activation promotes somatic cell reprogramming. Cell Res. 2013;23:131-41 pubmed publisher
	Lo Nigro A, de Jaime Soguero A, Khoueiry R, Cho D, Ferlazzo G, Perini I, et al. PDGFR?+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors. Stem Cell Reports. 2017;8:318-333 pubmed publisher
	Zhu L, Lv R, Kong L, Cheng H, Lan F, Li X. Genome-Wide Mapping of 5mC and 5hmC Identified Differentially Modified Genomic Regions in Late-Onset Severe Preeclampsia: A Pilot Study. PLoS ONE. 2015;10:e0134119 pubmed publisher
	Ivanov M, Kals M, Kacevska M, Barragan I, Kasuga K, Rane A, et al. Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function. Genome Biol. 2013;14:R83 pubmed publisher

product information

Catalog Number :

39649

Product Name :

5-Methylcytosine (5-mC) antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

50 ug

Clone :

33D3

Isotype :

IgG

CrossReactivity :

Other (Not Species Specific)

Background :

5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

Immunogen :

Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Uses :

DB, FACS, IHC, IP, MeDIP

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: Reynaud, C. and Nivelleau A. (1990) Annal. Letters 23: 31-45.Sasco, A.J. et. al. (1996) Cancer Letters 108: 157-162.Rougier, N. et. al. (1998) Genes and development 14: 2108-2113.Habib, M. et. al. (1999) Exp. Cell Res. 249: 46-53