Histone H2BK120ub1 antibody (mAb) | Active Motif 39623 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H2BK120ub1 antibody (mAb)

catalog :

39623

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

fission yeast, human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; fission yeast; fig 1e	Materne P, Vazquez E, Sanchez M, Yague Sanz C, Anandhakumar J, Migeot V, et al. Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus. elife. 2016;5: pubmed publisher
western blot; fission yeast	DeGennaro C, Alver B, Marguerat S, Stepanova E, Davis C, BÃ¤hler J, et al. Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast. Mol Cell Biol. 2013;33:4779-92 pubmed publisher
	Mbogning J, PagÃ© V, Burston J, Schwenger E, Fisher R, Schwer B, et al. Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification. Nucleic Acids Res. 2015;43:9766-75 pubmed publisher
	Mbogning J, Nagy S, Page V, Schwer B, Shuman S, Fisher R, et al. The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast. PLoS Genet. 2013;9:e1004029 pubmed publisher

product information

Catalog Number :

39623

Product Name :

Histone H2BK120ub1 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Ubiquitylated

Size :

100 ug

Clone :

Isotype :

IgG2a

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.

Immunogen :

This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.

Uses :

ChIP, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used as described in Minsky, N. et al. (2008) nat Cell Biol 10: 483M-bM-^@M-^S488.