PARP-1 N-terminal antibody (pAb) | Active Motif 39559 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

PARP-1 N-terminal antibody (pAb)

catalog :

39559

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay

citations: 4

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; human; fig 7	Watanabe Y, Papoutsoglou P, Maturi V, Tsubakihara Y, Hottiger M, Heldin C, et al. Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation. J Biol Chem. 2016;291:12706-23 pubmed publisher
western blot; mouse; fig 6	Myers S, Peddada S, Chatterjee N, Friedrich T, Tomoda K, Krings G, et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. elife. 2016;5:e10647 pubmed publisher
	Pandya P, Kublo L, Stewart Ornstein J. p53 Promotes Cytokine Expression in Melanoma to Regulate Drug Resistance and Migration. Cells. 2022;11: pubmed publisher
	Huang X, LeDuc R, Fornelli L, Schunter A, Bennett R, Kelleher N, et al. Defining the NSD2 interactome: PARP1 PARylation reduces NSD2 histone methyltransferase activity and impedes chromatin binding. J Biol Chem. 2019;: pubmed publisher

product information

Catalog Number :

39559

Product Name :

PARP-1 N-terminal antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human

Background :

PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.

Immunogen :

This PARP-1 N-terminal antibody was raised against a His-tagged fusion protein corresponding to the N-terminal half of human PARP-1 was used to generate this PARP-1 antibody.

Uses :

ChIP, IHC, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used in the following references: Kim MY et al. (2004) Cell 119: 803-814.Krishnakumar R et al. (2008) Science 319: 819-821.