Histone H3K27me2me3 antibody (mAb) | Active Motif 39535 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K27me2me3 antibody (mAb)

catalog :

39535

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat, roundworm

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, proximity ligation assay, blocking or activating experiments

citations: 28

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; rat; loading ...; fig 9b western blot; rat; loading ...; fig 1a	Jiang Y, Xiang C, Zhong F, Zhang Y, Wang L, Zhao Y, et al. Histone H3K27 methyltransferase EZH2 and demethylase JMJD3 regulate hepatic stellate cells activation and liver fibrosis. Theranostics. 2021;11:361-378 pubmed publisher
proximity ligation assay; human; loading ...; fig 4e immunocytochemistry; human; 1:500; loading ...; fig 3e	de Castro I, Budzak J, Di Giacinto M, Ligammari L, Gokhan E, Spanos C, et al. Repo-Man/PP1 regulates heterochromatin formation in interphase. Nat Commun. 2017;8:14048 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig s10	Faralli H, Wang C, Nakka K, Benyoucef A, Sebastian S, Zhuang L, et al. UTX demethylase activity is required for satellite cell-mediated muscle regeneration. J Clin Invest. 2016;126:1555-65 pubmed publisher
ChIP-Seq; mouse; fig 3a	Sin H, Kartashov A, Hasegawa K, Barski A, Namekawa S. Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline. BMC Biol. 2015;13:53 pubmed publisher
chromatin immunoprecipitation; human; fig 7	Heubach J, Monsior J, Deenen R, Niegisch G, Szarvas T, Niedworok C, et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer. 2015;14:108 pubmed publisher
chromatin immunoprecipitation; human	Balmer N, Klima S, Rempel E, Ivanova V, Kolde R, Weng M, et al. From transient transcriptome responses to disturbed neurodevelopment: role of histone acetylation and methylation as epigenetic switch between reversible and irreversible drug effects. Arch Toxicol. 2014;88:1451-68 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200	McLaughlin N, Wang F, Saifudeen Z, El Dahr S. In situ histone landscape of nephrogenesis. Epigenetics. 2014;9:222-35 pubmed publisher
immunohistochemistry; roundworm ; 1:30,000	Gaydos L, Rechtsteiner A, Egelhofer T, Carroll C, Strome S. Antagonism between MES-4 and Polycomb repressive complex 2 promotes appropriate gene expression in C. elegans germ cells. Cell Rep. 2012;2:1169-77 pubmed publisher
chromatin immunoprecipitation; mouse	Makeyev A, Enkhmandakh B, Hong S, Joshi P, Shin D, Bayarsaihan D. Diversity and complexity in chromatin recognition by TFII-I transcription factors in pluripotent embryonic stem cells and embryonic tissues. PLoS ONE. 2012;7:e44443 pubmed publisher
chromatin immunoprecipitation; human	Weng M, Zimmer B, Pöltl D, Broeg M, Ivanova V, Gaspar J, et al. Extensive transcriptional regulation of chromatin modifiers during human neurodevelopment. PLoS ONE. 2012;7:e36708 pubmed publisher
chromatin immunoprecipitation; human	Kloth M, Goering W, Ribarska T, Arsov C, Sorensen K, Schulz W. The SNP rs6441224 influences transcriptional activity and prognostically relevant hypermethylation of RARRES1 in prostate cancer. Int J Cancer. 2012;131:E897-904 pubmed publisher
chromatin immunoprecipitation; human	Murata T, Kondo Y, Sugimoto A, Kawashima D, Saito S, Isomura H, et al. Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells. J Virol. 2012;86:4752-61 pubmed publisher
immunocytochemistry; mouse	Sarma K, Levasseur P, Aristarkhov A, Lee J. Locked nucleic acids (LNAs) reveal sequence requirements and kinetics of Xist RNA localization to the X chromosome. Proc Natl Acad Sci U S A. 2010;107:22196-201 pubmed publisher
chromatin immunoprecipitation; human	Ribarska T, Ingenwerth M, Goering W, Engers R, Schulz W. Epigenetic inactivation of the placentally imprinted tumor suppressor gene TFPI2 in prostate carcinoma. Cancer Genomics Proteomics. 2010;7:51-60 pubmed
chromatin immunoprecipitation; human; 1:100	Menigatti M, Cattaneo E, Sabates Bellver J, Ilinsky V, Went P, Buffoli F, et al. The protein tyrosine phosphatase receptor type R gene is an early and frequent target of silencing in human colorectal tumorigenesis. Mol Cancer. 2009;8:124 pubmed publisher
	Schwartz Orbach L, Zhang C, Sidoli S, Amin R, Kaur D, Zhebrun A, et al. Caenorhabditis elegans nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3. elife. 2020;9: pubmed publisher
	Watanabe S, Shimada S, Akiyama Y, Ishikawa Y, Ogura T, Ogawa K, et al. Loss of KDM6A characterizes a poor prognostic subtype of human pancreatic cancer and potentiates HDAC inhibitor lethality. Int J Cancer. 2019;145:192-205 pubmed publisher
	Froberg J, Pinter S, Kriz A, Jégu T, Lee J. Megadomains and superloops form dynamically but are dispensable for X-chromosome inactivation and gene escape. Nat Commun. 2018;9:5004 pubmed publisher
	Jamieson K, Wiles E, McNaught K, Sidoli S, Leggett N, Shao Y, et al. Loss of HP1 causes depletion of H3K27me3 from facultative heterochromatin and gain of H3K27me2 at constitutive heterochromatin. Genome Res. 2016;26:97-107 pubmed publisher
	Rahman M, Munzig M, Kaneshiro K, Lee B, Strome S, MÃ¼ller Reichert T, et al. Caenorhabditis elegans polo-like kinase PLK-1 is required for merging parental genomes into a single nucleus. Mol Biol Cell. 2015;26:4718-35 pubmed publisher
	Pham K, Inoue Y, Vu B, Nguyen H, Nakayashiki T, Ikeda K, et al. MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis. PLoS Genet. 2015;11:e1005385 pubmed publisher
	Hannibal R, Chuong E, Rivera Mulia J, Gilbert D, Valouev A, Baker J. Copy number variation is a fundamental aspect of the placental genome. PLoS Genet. 2014;10:e1004290 pubmed publisher
	Connolly L, Smith K, Freitag M. The Fusarium graminearum histone H3 K27 methyltransferase KMT6 regulates development and expression of secondary metabolite gene clusters. PLoS Genet. 2013;9:e1003916 pubmed publisher
	Chuong E, Rumi M, Soares M, Baker J. Endogenous retroviruses function as species-specific enhancer elements in the placenta. Nat Genet. 2013;45:325-9 pubmed publisher
	Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
	Dhayalan A, Rajavelu A, Rathert P, Tamas R, Jurkowska R, Ragozin S, et al. The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation. J Biol Chem. 2010;285:26114-20 pubmed publisher
	Morin R, Johnson N, Severson T, Mungall A, An J, Goya R, et al. Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin. Nat Genet. 2010;42:181-5 pubmed publisher
	Baskind H, Na L, Ma Q, Patel M, Geenen D, Wang Q. Functional conservation of Asxl2, a murine homolog for the Drosophila enhancer of trithorax and polycomb group gene Asx. PLoS ONE. 2009;4:e4750 pubmed publisher

product information

Catalog Number :

39535

Product Name :

Histone H3K27me2me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

200 ul

Clone :

7B11

Isotype :

IgG

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or H3K27me3) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, IP, WB

Storage :

Antibodies in solution can be stored at -200C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Egelhofer T. et al. (2011) Nature Structural & Molecular Biology 18: 91-93.WB: Baskind H. et al. (2009) Plos One 4:e4750.