HDAC1 antibody (mAb) | Active Motif 39531 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

HDAC1 antibody (mAb)

catalog :

39531

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10E2

The same clone is also sold as:

ImmuQuest: IQ555
Cell Signaling Technology: 5356
Invitrogen: MA5-46593, MA5-18071
Santa Cruz Biotechnology: sc-81598

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; fig 3	Cohen I, Idan C, Rider P, Peleg R, Vornov E, Elena V, et al. IL-1Î± is a DNA damage sensor linking genotoxic stress signaling to sterile inflammation and innate immunity. Sci Rep. 2015;5:14756 pubmed publisher
western blot; mouse; 1:500	Tyler C, Hafez A, Solomon E, Allan A. Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain. Toxicol Appl Pharmacol. 2015;288:40-51 pubmed publisher
chromatin immunoprecipitation; rat	Winston J, Li Q, Sarna S. Chronic prenatal stress epigenetically modifies spinal cord BDNF expression to induce sex-specific visceral hypersensitivity in offspring. Neurogastroenterol Motil. 2014;26:715-30 pubmed publisher

product information

Catalog Number :

39531

Product Name :

HDAC1 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

200 ul

Clone :

10E2

Isotype :

IgG1

CrossReactivity :

Human, Mouse, Rat

Background :

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the M-NM-5-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Immunogen :

This HDAC1 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1.

Uses :

ChIP, IF, IHC, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used in Lagger et al. (2002) EMBO J 21:2672 and in Lagger et al. (2003) Mol Cell Biol 23:2669.