Histone H2AT120ph antibody (pAb) | Active Motif 39391 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H2AT120ph antibody (pAb)

catalog :

39391

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:2500; loading ...; fig 2c	Akera T, Trimm E, Lampson M. Molecular Strategies of Meiotic Cheating by Selfish Centromeres. Cell. 2019;178:1132-1144.e10 pubmed publisher
immunocytochemistry; human; 1:2000; loading ...; fig s7e	Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
	Lee H, Lin Z, Chae S, Yoo Y, Kim B, Lee Y, et al. The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nat Commun. 2018;9:3848 pubmed publisher

product information

Catalog Number :

39391

Product Name :

Histone H2AT120ph antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure.

Immunogen :

This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho-threonine 120 of histone H2A.

Uses :

DB, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.