Histone H3K4me1 antibody (pAb) | Active Motif 39297 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K4me1 antibody (pAb)

catalog :

39297

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, zebrafish , fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 14

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; 1:250; fig 1a	Reddy N, Majidi S, Kong L, Nemera M, Ferguson C, Moore M, et al. CHARGE syndrome protein CHD7 regulates epigenomic activation of enhancers in granule cell precursors and gyrification of the cerebellum. Nat Commun. 2021;12:5702 pubmed publisher
chromatin immunoprecipitation; mouse; 1:500; loading ...; fig s7f, s7g	Cooper A, Butto T, Hammer N, Jagannath S, Fend Guella D, Akhtar J, et al. Inhibition of histone deacetylation rescues phenotype in a mouse model of Birk-Barel intellectual disability syndrome. Nat Commun. 2020;11:480 pubmed publisher
chromatin immunoprecipitation; human; fig s4b	Wu S, Turner K, Nguyen N, Raviram R, Erb M, Santini J, et al. Circular ecDNA promotes accessible chromatin and high oncogene expression. Nature. 2019;575:699-703 pubmed publisher
ChIP-Seq; human; loading ...; fig 5b	Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
ChIP-Seq; zebrafish ; fig s1a	Murphy P, Wu S, James C, Wike C, Cairns B. Placeholder Nucleosomes Underlie Germline-to-Embryo DNA Methylation Reprogramming. Cell. 2018;172:993-1006.e13 pubmed publisher
immunohistochemistry; fruit fly ; 1:100; loading ...; tbl s2	Zenk F, Loeser E, Schiavo R, Kilpert F, BogdanoviÄ‡ O, Iovino N. Germ line-inherited H3K27me3 restricts enhancer function during maternal-to-zygotic transition. Science. 2017;357:212-216 pubmed publisher
ChIP-Seq; mouse; loading ...; fig 1b	Cruz Molina S, Respuela P, Tebartz C, Kolovos P, Nikolic M, Fueyo R, et al. PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation. Cell Stem Cell. 2017;20:689-705.e9 pubmed publisher
ChIP-Seq; mouse; loading ...; fig 1c	Hu Y, Zhang Z, Kashiwagi M, Yoshida T, Joshi I, Jena N, et al. Superenhancer reprogramming drives a B-cell-epithelial transition and high-risk leukemia. Genes Dev. 2016;30:1971-90 pubmed publisher
western blot; mouse; fig 2	Carofino B, Ayanga B, Tracey L, Brooke Bisschop T, Justice M. PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL. Biol Open. 2016;5:645-53 pubmed publisher
ChIP-Seq; human; fig 3	Lu F, Chen H, Kossenkov A, DeWispeleare K, Won K, Lieberman P. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jκ and EBF1. PLoS Pathog. 2016;12:e1005339 pubmed publisher
	Li M, Huang H, Li L, He C, Zhu L, Guo H, et al. Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis. Nat Commun. 2021;12:420 pubmed publisher
	Huang Y, Mouttet B, Warnatz H, Risch T, Rietmann F, Frommelt F, et al. The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular Identity and Self-Renewal Conferring EP300 Vulnerability. Cancer Cell. 2019;36:630-644.e9 pubmed publisher
	Wohlfahrt T, Rauber S, Uebe S, Luber M, Soare A, Ekici A, et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature. 2019;566:344-349 pubmed publisher
	Lakshminarasimhan R, Andreu Vieyra C, Lawrenson K, Duymich C, Gayther S, Liang G, et al. Down-regulation of ARID1A is sufficient to initiate neoplastic transformation along with epigenetic reprogramming in non-tumorigenic endometriotic cells. Cancer Lett. 2017;401:11-19 pubmed publisher

product information

Catalog Number :

39297

Product Name :

Histone H3K4me1 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Immunogen :

This Histone H3 monomethyl Lys4 antibody was raised against a peptide including monomethyl-lysine 4 of human histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, ELISA, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Egelhofer T. et al. (2011) Nature Structural & Molecular Biology 18: 91-93.