Histone H3K9me3 antibody (mAb) | Active Motif 39285 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K9me3 antibody (mAb)

catalog :

39285

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2AG-6F12-H4

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:100; fig 6	Popken J, Dahlhoff M, Guengoer T, Wolf E, Zakhartchenko V. 3D structured illumination microscopy of mammalian embryos and spermatozoa. BMC Dev Biol. 2015;15:46 pubmed publisher
immunohistochemistry; human; loading ...; fig 7a	HÃ¼bner B, Lomiento M, Mammoli F, Illner D, Markaki Y, Ferrari S, et al. Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments. Epigenetics Chromatin. 2015;8:47 pubmed publisher
immunocytochemistry; mouse; 1:100 western blot; human	Liu H, Galka M, Iberg A, Wang Z, Li L, Voss C, et al. Systematic identification of methyllysine-driven interactions for histone and nonhistone targets. J Proteome Res. 2010;9:5827-36 pubmed publisher
	Papazyan R, Voronina E, Chapman J, Luperchio T, Gilbert T, Meier E, et al. Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis. elife. 2014;3:e02996 pubmed publisher

product information

Catalog Number :

39285

Product Name :

Histone H3K9me3 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Antigen Modification :

Methylated

Size :

200 ul

Clone :

2AG-6F12-H4

Isotype :

IgG1k

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 trimethyl Lys9 antibody was raised against a peptide containing trimethyl-lysineM-BM- 9 of human histone H3.

Uses :

DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.