Histone H3K56ac antibody (pAb) | Active Motif 39281 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K56ac antibody (pAb)

catalog :

39281

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 4b	Liang X, Yuan X, Yu J, Wu Y, Li K, Sun C, et al. Histone Chaperone ASF1A Predicts Poor Outcomes for Patients With Gastrointestinal Cancer and Drives Cancer Progression by Stimulating Transcription of β-Catenin Target Genes. EBioMedicine. 2017;21:104-116 pubmed publisher
western blot; human; loading ...; fig 6a	Meisenberg C, Ashour M, El Shafie L, Liao C, Hodgson A, Pilborough A, et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res. 2017;45:1159-1176 pubmed publisher
western blot; human; loading ...; fig 2a	Pal S, Graves H, Ohsawa R, Huang T, Wang P, Harmacek L, et al. The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells. PLoS ONE. 2016;11:e0155409 pubmed publisher
	Gomes A, Ilter D, Low V, Endress J, Fernández García J, Rosenzweig A, et al. Age-induced accumulation of methylmalonic acid promotes tumour progression. Nature. 2020;585:283-287 pubmed publisher
	Rezazadeh S, Yang D, Tombline G, Simon M, Regan S, Seluanov A, et al. SIRT6 promotes transcription of a subset of NRF2 targets by mono-ADP-ribosylating BAF170. Nucleic Acids Res. 2019;: pubmed publisher

product information

Catalog Number :

39281

Product Name :

Histone H3K56ac antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Acetylated

Size :

200 ul

Isotype :

IgG

CrossReactivity :

Budding Yeast, Human

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

Immunogen :

This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.