Histone H3K36me2 antibody (pAb) | Active Motif 39255 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K36me2 antibody (pAb)

catalog :

39255

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, brewer's yeast

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; 1:5000; loading ...; fig 1a	Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher
western blot; human; fig 2	García Carpizo V, Sarmentero J, Han B, Grana O, Ruiz Llorente S, Pisano D, et al. NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation. Sci Rep. 2016;6:32952 pubmed publisher
	Dronamraju R, Jha D, Eser U, Adams A, Dominguez D, Choudhury R, et al. Set2 methyltransferase facilitates cell cycle progression by maintaining transcriptional fidelity. Nucleic Acids Res. 2018;46:1331-1344 pubmed publisher

product information

Catalog Number :

39255

Product Name :

Histone H3K36me2 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Budding Yeast, Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Immunogen :

This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.