Histone H3K9me2 antibody (pAb) | Active Motif 39239 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K9me2 antibody (pAb)

catalog :

39239

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig s7a	Lee J, Termglinchan V, Diecke S, Itzhaki I, Lam C, Garg P, et al. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy. Nature. 2019;572:335-340 pubmed publisher
chromatin immunoprecipitation; human; fig 4	Brasa S, Mueller A, Jacquemont S, Hahne F, Rozenberg I, Peters T, et al. Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome. Clin Epigenetics. 2016;8:15 pubmed publisher
immunocytochemistry; mouse; 1:800; fig 2	Ancelin K, Syx L, Borensztein M, Ranisavljevic N, Vassilev I, Briseño Roa L, et al. Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation. elife. 2016;5: pubmed publisher
	Poleshko A, Smith C, Nguyen S, Sivaramakrishnan P, Wong K, Murray J, et al. H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis. elife. 2019;8: pubmed publisher
	Valsecchi C, Basilicata M, Semplicio G, Georgiev P, Gutierrez N, Akhtar A. Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells. Nat Commun. 2018;9:3626 pubmed publisher
	Poleshko A, Shah P, Gupta M, Babu A, Morley M, Manderfield L, et al. Genome-Nuclear Lamina Interactions Regulate Cardiac Stem Cell Lineage Restriction. Cell. 2017;171:573-587.e14 pubmed publisher
	Mursalimov S, Permyakova N, Deineko E, Houben A, Demidov D. Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes. Front Plant Sci. 2015;6:846 pubmed publisher

product information

Catalog Number :

39239

Product Name :

Histone H3K9me2 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human, Mouse, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 dimethyl Lys9 antibody was raised against a peptide including dimethyl-lysine 9 of histone H3.

Uses :

ChIP, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.