RNA pol II CTD phospho Ser5 antibody (pAb) | Active Motif 39233 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA pol II CTD phospho Ser5 antibody (pAb)

catalog :

39233

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; fig 5	Malecova B, Dall Agnese A, Madaro L, Gatto S, Coutinho Toto P, Albini S, et al. TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells. elife. 2016;5: pubmed publisher

product information

Catalog Number :

39233

Product Name :

RNA pol II CTD phospho Ser5 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Phosphorylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human, Other (Wide Range)

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Immunogen :

This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Uses :

ChIP, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.