DNMT1 antibody (mAb) | Active Motif 39204 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

DNMT1 antibody (mAb)

catalog :

39204

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

60B1220.1

The same clone is also sold as:

Abnova: MAB0079
StressMarq Biosciences: SMC-200
Invitrogen: MA5-16169, MA5-45293, MA5-45291, MA5-45292
MyBioSource: MBS245208
Novus Biologicals: NBP2-80697, NB100-56519
OriGene: TA326439, TA336382
Rockland Immunochemicals: 200-301-H69
Abcam: ab13537

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 3	Hervouet E, Claude Taupin A, Gauthier T, Perez V, Fraichard A, Adami P, et al. The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models. BMC Cancer. 2015;15:729 pubmed publisher
western blot; mouse; fig 1,6,7 western blot; human; fig 1,6,7	Liao J, Karnik R, Gu H, Ziller M, Clement K, Tsankov A, et al. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Nat Genet. 2015;47:469-78 pubmed publisher
western blot; mouse	Singh A, Archer T. Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation. Nucleic Acids Res. 2014;42:2958-75 pubmed publisher
western blot; mouse; 1:1000	Takasugi M, Hayakawa K, Arai D, Shiota K. Age- and sex-dependent DNA hypomethylation controlled by growth hormone in mouse liver. Mech Ageing Dev. 2013;134:331-7 pubmed publisher
western blot; mouse; 1:2000	Wiench M, John S, Baek S, Johnson T, Sung M, Escobar T, et al. DNA methylation status predicts cell type-specific enhancer activity. EMBO J. 2011;30:3028-39 pubmed publisher
chromatin immunoprecipitation; human western blot; human	Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher
chromatin immunoprecipitation; mouse	Schnekenburger M, Talaska G, Puga A. Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation. Mol Cell Biol. 2007;27:7089-101 pubmed
	Zhao X, Hu X. Downregulated long noncoding RNA LINC00313 inhibits the epithelial-mesenchymal transition, invasion, and migration of thyroid cancer cells through inhibiting the methylation of ALX4. J Cell Physiol. 2019;: pubmed publisher
	Galonska C, Charlton J, Mattei A, Donaghey J, Clement K, Gu H, et al. Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun. 2018;9:597 pubmed publisher

product information

Catalog Number :

39204

Product Name :

DNMT1 antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

100 ug

Clone :

60B1220.1

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

DNMT1 M-bM-^@M-^S DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Immunogen :

This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.

Uses :

ChIP, IHC, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody was used in ChIP and in Re-ChIP assays as described in Schnekenburger, M. et. al. (2007) Mol Cell Biol. 27(20): 7089M-bM-^@M-^S7101.