Histone H4K16ac antibody (pAb) | Active Motif 39167 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H4K16ac antibody (pAb)

catalog :

39167

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; loading ...; fig 6a	Wang Z, Millard C, Lin C, Gurnett J, Wu M, Lee K, et al. Diverse nucleosome site-selectivity among histone deacetylase complexes. elife. 2020;9: pubmed publisher
	Martire S, Nguyen J, Sundaresan A, Banaszynski L. Differential contribution of p300 and CBP to regulatory element acetylation in mESCs. BMC Mol Cell Biol. 2020;21:55 pubmed publisher
	De Ioannes P, Leon V, Kuang Z, Wang M, Boeke J, Hochwagen A, et al. Structure and function of the Orc1 BAH-nucleosome complex. Nat Commun. 2019;10:2894 pubmed publisher
	Urdinguio R, Lopez V, Bayon G, Díaz de la Guardia R, Sierra M, García Toraño E, et al. Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment. Nucleic Acids Res. 2019;47:5016-5037 pubmed publisher
	Copur Ö, Gorchakov A, Finkl K, Kuroda M, Muller J. Sex-specific phenotypes of histone H4 point mutants establish dosage compensation as the critical function of H4K16 acetylation in Drosophila. Proc Natl Acad Sci U S A. 2018;115:13336-13341 pubmed publisher
	Basilicata M, Bruel A, Semplicio G, Valsecchi C, Aktas T, Duffourd Y, et al. De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. Nat Genet. 2018;50:1442-1451 pubmed publisher
	Cohen A, Habib A, Laor D, Yadav S, Kupiec M, Weisman R. TOR complex 2 in fission yeast is required for chromatin-mediated gene silencing and assembly of heterochromatic domains at subtelomeres. J Biol Chem. 2018;293:8138-8150 pubmed publisher
	Korb E, Herre M, Zucker Scharff I, Gresack J, Allis C, Darnell R. Excess Translation of Epigenetic Regulators Contributes to Fragile X Syndrome and Is Alleviated by Brd4 Inhibition. Cell. 2017;170:1209-1223.e20 pubmed publisher
	Jih G, Iglesias N, Currie M, Bhanu N, Paulo J, Gygi S, et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature. 2017;547:463-467 pubmed publisher
	Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
	Freire Benéitez V, Price R, Buscaino A. The Chromatin of Candida albicans Pericentromeres Bears Features of Both Euchromatin and Heterochromatin. Front Microbiol. 2016;7:759 pubmed publisher
	Freire BenÃ©itez V, Price R, Tarrant D, Berman J, Buscaino A. Candida albicans repetitive elements display epigenetic diversity and plasticity. Sci Rep. 2016;6:22989 pubmed publisher

product information

Catalog Number :

39167

Product Name :

Histone H4K16ac antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Acetylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Budding Yeast, Drosophila, Human, Other (Wide Range)

Background :

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. NoRC is a SNF2h-containing chromatin remodeling complex. The bromodomain of TIP5, the large subunit of NoRC, interacts with acetylated Histone H4 Lys16, (H4AcK16) and cooperates with an adjacent PHD finger to recruit histone deacetylases and DNA methyltransferases to rDNA, leading to the silencing of rDNA.

Immunogen :

This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, IP, WB

Storage :

Antibodies in solution can be stored at -200C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Egelhofer T. et al. (2011) Nature Structural & Molecular Biology 18: 91-93.