Histone H4K12ac antibody (pAb) | Active Motif 39165 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H4K12ac antibody (pAb)

catalog :

39165

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, brewer's yeast

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; loading ...; fig s1b	Desfossés Baron K, Hammond Martel I, Simoneau A, Sellam A, Roberts S, Wurtele H. Valproate inhibits MAP kinase signalling and cell cycle progression in S. cerevisiae. Sci Rep. 2016;6:36013 pubmed publisher
western blot; human; fig 3a	Kehrli K, Phelps M, Lazarchuk P, Chen E, Monnat R, Sidorova J. Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest. J Biol Chem. 2016;291:24487-24503 pubmed
	Mustafi S, Camarena V, Volmar C, Huff T, Sant D, Brothers S, et al. Vitamin C Sensitizes Melanoma to BET Inhibitors. Cancer Res. 2018;78:572-583 pubmed publisher

product information

Catalog Number :

39165

Product Name :

Histone H4K12ac antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Acetylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Budding Yeast, Human, Other (Wide Range)

Background :

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation.

Immunogen :

This Histone H4 acetyl Lys12 antibody was raised against a peptide including acetyl-lysine 12 of histone H4.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, IF, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Egelhofer T. et al. (2011) Nature Structural & Molecular Biology 18: 91-93.WB: Tjeertes, J. et al. (2009) EMBO J 28: 1878M-bM-^@M-^S1889.