Histone H3, C-terminal antibody (pAb) | Active Motif 39163 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3, C-terminal antibody (pAb)

catalog :

39163

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, brewer's yeast

application :

western blot, ELISA, immunoprecipitation, chromatin immunoprecipitation

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1h	Li W, Wang H, Zhao X, Duan H, Cheng B, Liu Y, et al. A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair. Sci Adv. 2019;5:eaau7566 pubmed publisher
western blot; brewer's yeast; loading ...; fig 1a	Young C, Hillyer C, Hokamp K, Fitzpatrick D, Konstantinov N, Welty J, et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics. 2017;18:107 pubmed publisher
western blot; human; 1:1000; loading ...; fig s4b	de Castro I, Budzak J, Di Giacinto M, Ligammari L, Gokhan E, Spanos C, et al. Repo-Man/PP1 regulates heterochromatin formation in interphase. Nat Commun. 2017;8:14048 pubmed publisher
chromatin immunoprecipitation; rat; loading ...; fig 2b	Yang Y, Yamada T, Hill K, Hemberg M, Reddy N, Cho H, et al. Chromatin remodeling inactivates activity genes and regulates neural coding. Science. 2016;353:300-305 pubmed publisher
western blot; human; loading ...; fig 4	Liu Z, Lam N, Wang E, Virden R, Pawel B, Attiyeh E, et al. Identification of CASZ1 NES reveals potential mechanisms for loss of CASZ1 tumor suppressor activity in neuroblastoma. Oncogene. 2017;36:97-109 pubmed publisher
western blot; mouse; fig 1	Sheikh B, Bechtel Walz W, Lucci J, Karpiuk O, Hild I, Hartleben B, et al. MOF maintains transcriptional programs regulating cellular stress response. Oncogene. 2016;35:2698-710 pubmed publisher
	Uchiyama R, Kupkova K, Shetty S, Linford A, Pray Grant M, Wagar L, et al. Histone H3 lysine 4 methylation signature associated with human undernutrition. Proc Natl Acad Sci U S A. 2018;115:E11264-E11273 pubmed publisher
	Janevska S, Guldener U, Sulyok M, Tudzynski B, Studt L. Set1 and Kdm5 are antagonists for H3K4 methylation and regulators of the major conidiation-specific transcription factor gene ABA1 in Fusarium fujikuroi. Environ Microbiol. 2018;20:3343-3362 pubmed publisher
	Janevska S, Baumann L, Sieber C, Munsterkotter M, Ulrich J, Kämper J, et al. Elucidation of the Two H3K36me3 Histone Methyltransferases Set2 and Ash1 in Fusarium fujikuroi Unravels Their Different Chromosomal Targets and a Major Impact of Ash1 on Genome Stability. Genetics. 2018;208:153-171 pubmed publisher
	Hepp M, Smolle M, Gidi C, Amigo R, Valenzuela N, Arriagada A, et al. Role of Nhp6 and Hmo1 in SWI/SNF occupancy and nucleosome landscape at gene regulatory regions. Biochim Biophys Acta Gene Regul Mech. 2017;1860:316-326 pubmed publisher
	Akiyama Y, Koda Y, Byeon S, Shimada S, Nishikawaji T, Sakamoto A, et al. Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression. Oncotarget. 2016;7:3966-83 pubmed publisher

product information

Catalog Number :

39163

Product Name :

Histone H3, C-terminal antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Budding Yeast, Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Immunogen :

This Histone H3 C-terminal antibody was raised against a C-terminal peptide of histone H3.

Uses :

ChIP, ELISA, IP, WB

Storage :

Antibodies in solution can be stored at -200C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: Baskind et al. (2009) Plos One 4:e4750.