Histone H3K9me3 antibody (pAb) | Active Motif 39161 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K9me3 antibody (pAb)

catalog :

39161

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, fruit fly

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 31

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 5d	Yi S, Jang Y, Kim H, Lee K, Lee H, Kim Y, et al. The KDM4B-CCAR1-MED1 axis is a critical regulator of osteoclast differentiation and bone homeostasis. Bone Res. 2021;9:27 pubmed publisher
western blot; human; fig 1e	Andrade J, Shi C, Costa A, Choi J, Kim J, Doddaballapur A, et al. Control of endothelial quiescence by FOXO-regulated metabolites. Nat Cell Biol. 2021;23:413-423 pubmed publisher
ChIP-Seq; fruit fly ; loading ...; fig 1s2h	Fabry M, Ciabrelli F, Munafò M, Eastwood E, Kneuss E, Falciatori I, et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. elife. 2019;8: pubmed publisher
ChIP-Seq; human; loading ...; fig 5b	Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
immunocytochemistry; human; 1:500; loading ...; fig 5b	Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, et al. Identification of the elementary structural units of the DNA damage response. Nat Commun. 2017;8:15760 pubmed publisher
western blot; human; fig 1	Jayaram H, Hoelper D, Jain S, Cantone N, Lundgren S, Poy F, et al. S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3. Proc Natl Acad Sci U S A. 2016;113:6182-7 pubmed publisher
chromatin immunoprecipitation; human; fig 1	Dheekollu J, Wiedmer A, Sentana Lledo D, Cassel J, Messick T, Lieberman P. HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus. J Virol. 2016;90:5353-5367 pubmed publisher
	Novo C, Wong E, Hockings C, Poudel C, Sheekey E, Wiese M, et al. Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells. Nat Commun. 2022;13:3525 pubmed publisher
	Liang Y, Fan Y, Liu Y, Fan H. HNRNPU promotes the progression of hepatocellular carcinoma by enhancing CDK2 transcription. Exp Cell Res. 2021;409:112898 pubmed publisher
	Das S, Chadwick B. CRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy. Sci Rep. 2021;11:12598 pubmed publisher
	Fukuda K, Shimura C, Miura H, Tanigawa A, Suzuki T, Dohmae N, et al. Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases. Commun Biol. 2021;4:571 pubmed publisher
	Fitz James M, Tong P, Pidoux A, Özadam H, Yang L, White S, et al. Large domains of heterochromatin direct the formation of short mitotic chromosome loops. elife. 2020;9: pubmed publisher
	Wolf G, De Iaco A, Sun M, Bruno M, Tinkham M, Hoang D, et al. KRAB-zinc finger protein gene expansion in response to active retrotransposons in the murine lineage. elife. 2020;9: pubmed publisher
	Roy A, Ghosh A, Kumar B, Chandran B. IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP. elife. 2019;8: pubmed publisher
	Wang S, Zhang C, Hasson D, Desai A, SenBanerjee S, Magnani E, et al. Epigenetic Compensation Promotes Liver Regeneration. Dev Cell. 2019;: pubmed publisher
	Wohlfahrt T, Rauber S, Uebe S, Luber M, Soare A, Ekici A, et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature. 2019;566:344-349 pubmed publisher
	Fenizia C, Bottino C, Corbetta S, Fittipaldi R, Floris P, Gaudenzi G, et al. SMYD3 promotes the epithelial-mesenchymal transition in breast cancer. Nucleic Acids Res. 2019;47:1278-1293 pubmed publisher
	Hirota T, Blakeley P, Sangrithi M, Mahadevaiah S, Encheva V, Snijders A, et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell. 2018;47:645-659.e6 pubmed publisher
	Chen X, Zhang M, Gan H, Wang H, Lee J, Fang D, et al. A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Nat Commun. 2018;9:2949 pubmed publisher
	Bonnet Garnier A, Kiêu K, Aguirre Lavin T, Tar K, Flores P, Liu Z, et al. Three-dimensional analysis of nuclear heterochromatin distribution during early development in the rabbit. Chromosoma. 2018;127:387-403 pubmed publisher
	Pavlaki I, Alammari F, Sun B, Clark N, Sirey T, Lee S, et al. The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. EMBO J. 2018;37: pubmed publisher
	Chiarella A, Quimby A, Mehrab Mohseni M, Velasco B, Kasoji S, Davis I, et al. Cavitation Enhancement Increases the Efficiency and Consistency of Chromatin Fragmentation from Fixed Cells for Downstream Quantitative Applications. Biochemistry. 2018;57:2756-2761 pubmed publisher
	Balog J, Goossens R, Lemmers R, Straasheijm K, van der Vliet P, Heuvel A, et al. Monosomy 18p is a risk factor for facioscapulohumeral dystrophy. J Med Genet. 2018;55:469-478 pubmed publisher
	Niskanen H, Tuszynska I, Zaborowski R, Heinaniemi M, Yla Herttuala S, Wilczynski B, et al. Endothelial cell differentiation is encompassed by changes in long range interactions between inactive chromatin regions. Nucleic Acids Res. 2017;: pubmed publisher
	Gessaman J, Selker E. Induction of H3K9me3 and DNA methylation by tethered heterochromatin factors in Neurospora crassa. Proc Natl Acad Sci U S A. 2017;114:E9598-E9607 pubmed publisher
	Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A. Modular fluorescence complementation sensors for live cell detection of epigenetic signals at endogenous genomic sites. Nat Commun. 2017;8:649 pubmed publisher
	Schorn A, Gutbrod M, Leblanc C, Martienssen R. LTR-Retrotransposon Control by tRNA-Derived Small RNAs. Cell. 2017;170:61-71.e11 pubmed publisher
	Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, et al. The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis. Neuron. 2017;94:550-568.e10 pubmed publisher
	Das D, Sengupta I, Sarkar N, Pal A, Saha D, Bandopadhyay M, et al. Anti-hepatitis B virus (HBV) response of imiquimod based toll like receptor 7 ligand in hbv-positive human hepatocelluar carcinoma cell line. BMC Infect Dis. 2017;17:76 pubmed publisher
	Chen X, Rinsma M, Janssen J, Liu J, Maggio I, Gonçalves M. Probing the impact of chromatin conformation on genome editing tools. Nucleic Acids Res. 2016;44:6482-92 pubmed publisher
	Jamieson K, Wiles E, McNaught K, Sidoli S, Leggett N, Shao Y, et al. Loss of HP1 causes depletion of H3K27me3 from facultative heterochromatin and gain of H3K27me2 at constitutive heterochromatin. Genome Res. 2016;26:97-107 pubmed publisher

product information

Catalog Number :

39161

Product Name :

Histone H3K9me3 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Fission Yeast, Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, ELISA, IF, IP, WB

Storage :

Antibodies in solution can be stored at -200C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Karimi M. et al. (2011) Cell Stem Cell 8(6):676-687.ChIP: Rai K. et al. (2010) J Biol Chem 285(6): 4110-4121.