Histone H3K4me2 antibody (pAb) | Active Motif 39141 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K4me2 antibody (pAb)

catalog :

39141

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:100; loading ...; fig s2a	Kuhn T, Pascual García P, Gozalo A, Little S, Capelson M. Chromatin targeting of nuclear pore proteins induces chromatin decondensation. J Cell Biol. 2019;218:2945-2961 pubmed publisher
ChIP-Seq; human; loading ...; fig 5b	Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 4c immunohistochemistry; human; fig s6	Sareddy G, Viswanadhapalli S, Surapaneni P, Suzuki T, Brenner A, Vadlamudi R. Novel KDM1A inhibitors induce differentiation and apoptosis of glioma stem cells via unfolded protein response pathway. Oncogene. 2017;36:2423-2434 pubmed publisher
chromatin immunoprecipitation; human; fig 1	Dheekollu J, Wiedmer A, Sentana Lledo D, Cassel J, Messick T, Lieberman P. HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus. J Virol. 2016;90:5353-5367 pubmed publisher
	Pinter S, Knodel F, Choudalakis M, Schnee P, Kroll C, Fuchs M, et al. A functional LSD1 coregulator screen reveals a novel transcriptional regulatory cascade connecting R-loop homeostasis with epigenetic regulation. Nucleic Acids Res. 2021;49:4350-4370 pubmed publisher
	Janevska S, Guldener U, Sulyok M, Tudzynski B, Studt L. Set1 and Kdm5 are antagonists for H3K4 methylation and regulators of the major conidiation-specific transcription factor gene ABA1 in Fusarium fujikuroi. Environ Microbiol. 2018;20:3343-3362 pubmed publisher
	Balog J, Goossens R, Lemmers R, Straasheijm K, van der Vliet P, Heuvel A, et al. Monosomy 18p is a risk factor for facioscapulohumeral dystrophy. J Med Genet. 2018;55:469-478 pubmed publisher
	Freire Benéitez V, Price R, Buscaino A. The Chromatin of Candida albicans Pericentromeres Bears Features of Both Euchromatin and Heterochromatin. Front Microbiol. 2016;7:759 pubmed publisher
	Freire BenÃ©itez V, Price R, Tarrant D, Berman J, Buscaino A. Candida albicans repetitive elements display epigenetic diversity and plasticity. Sci Rep. 2016;6:22989 pubmed publisher
	Gallardo Vara E, Blanco F, RoquÃ© M, Friedman S, Suzuki T, Botella L, et al. Transcription factor KLF6 upregulates expression of metalloprotease MMP14 and subsequent release of soluble endoglin during vascular injury. Angiogenesis. 2016;19:155-71 pubmed publisher

product information

Catalog Number :

39141

Product Name :

Histone H3K4me2 antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Methylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Immunogen :

This Histone H3 dimethyl Lys4 antibody was raised against a peptide including dimethyl-lysine 4 of histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, ELISA, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.