Histone H3K27ac antibody (pAb) | Active Motif 39135 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

Histone H3K27ac antibody (pAb)

catalog :

39135

quantity :

200 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, fruit fly

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly ; 1:100; loading ...; fig 2b	Kuhn T, Pascual García P, Gozalo A, Little S, Capelson M. Chromatin targeting of nuclear pore proteins induces chromatin decondensation. J Cell Biol. 2019;218:2945-2961 pubmed publisher
ChIP-Seq; human; loading ...; fig 3b	Nava M, Dutta P, Zemke N, Farias Eisner R, Vadgama J, Wu Y. Transcriptomic and ChIP-sequence interrogation of EGFR signaling in HER2+ breast cancer cells reveals a dynamic chromatin landscape and S100 genes as targets. BMC Med Genomics. 2019;12:32 pubmed publisher
ChIP-Seq; human; loading ...; fig s9b ChIP-Seq; mouse; loading ...; fig s9f	Gosselin D, Skola D, Coufal N, Holtman I, Schlachetzki J, Sajti E, et al. An environment-dependent transcriptional network specifies human microglia identity. Science. 2017;356: pubmed publisher
ChIP-Seq; mouse; fig 5	Eichenfield D, Troutman T, Link V, Lam M, Cho H, Gosselin D, et al. Tissue damage drives co-localization of NF-?B, Smad3, and Nrf2 to direct Rev-erb sensitive wound repair in mouse macrophages. elife. 2016;5: pubmed publisher
ChIP-Seq; mouse; loading ...; fig 5d	Allison K, Sajti E, Collier J, Gosselin D, Troutman T, Stone E, et al. Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells. elife. 2016;5: pubmed publisher
	Mayer D, Stadler M, Rittirsch M, Hess D, Lukonin I, Winzi M, et al. Zfp281 orchestrates interconversion of pluripotent states by engaging Ehmt1 and Zic2. EMBO J. 2019;:e102591 pubmed publisher
	Anastas J, Zee B, Kalin J, Kim M, Guo R, Alexandrescu S, et al. Re-programing Chromatin with a Bifunctional LSD1/HDAC Inhibitor Induces Therapeutic Differentiation in DIPG. Cancer Cell. 2019;36:528-544.e10 pubmed publisher
	Dall Agnese A, Caputo L, Nicoletti C, Di Iulio J, SCHMITT A, Gatto S, et al. Transcription Factor-Directed Re-wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming toward trans-Differentiation. Mol Cell. 2019;76:453-472.e8 pubmed publisher
	Kalkan T, Bornelöv S, Mulas C, Diamanti E, Lohoff T, Ralser M, et al. Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency. Cell Stem Cell. 2019;24:785-801.e7 pubmed publisher
	Wohlfahrt T, Rauber S, Uebe S, Luber M, Soare A, Ekici A, et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature. 2019;566:344-349 pubmed publisher
	Watanabe S, Shimada S, Akiyama Y, Ishikawa Y, Ogura T, Ogawa K, et al. Loss of KDM6A characterizes a poor prognostic subtype of human pancreatic cancer and potentiates HDAC inhibitor lethality. Int J Cancer. 2019;145:192-205 pubmed publisher
	Hulse M, Caruso L, Madzo J, Tan Y, Johnson S, Tempera I. Poly(ADP-ribose) polymerase 1 is necessary for coactivating hypoxia-inducible factor-1-dependent gene expression by Epstein-Barr virus latent membrane protein 1. PLoS Pathog. 2018;14:e1007394 pubmed publisher
	Zhao C, Dong C, Frah M, Deng Y, Marie C, Zhang F, et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell. 2018;45:753-768.e8 pubmed publisher
	Link V, Duttke S, Chun H, Holtman I, Westin E, Hoeksema M, et al. Analysis of Genetically Diverse Macrophages Reveals Local and Domain-wide Mechanisms that Control Transcription Factor Binding and Function. Cell. 2018;173:1796-1809.e17 pubmed publisher

product information

Catalog Number :

39135

Product Name :

Histone H3K27ac antibody (pAb)

Host Species :

Rabbit

Clonality :

Polyclonal

Antigen Modification :

Acetylated

Size :

200 ul

Isotype :

Serum

CrossReactivity :

Budding Yeast, Human, Other (Wide Range)

Background :

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.Lysine N-M-NM-5-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation.Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Immunogen :

This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.

Uses :

ChIP, ChIP-chip, ChIP-Seq, DB, IP, WB

Storage :

Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Product References :

References: This antibody has been used as described below:ChIP-Seq: Egelhofer T. et al. (2011) Nature Structural & Molecular Biology 18: 91-93.