RNA pol II antibody (mAb) | Active Motif 39097 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Active Motif

other brands :

Geneka Biotechnology

product type :

antibody

product name :

RNA pol II antibody (mAb)

catalog :

39097

quantity :

200 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

hamsters, human, mouse, rat, chicken, roundworm

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 26

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; 1:100; fig 5??s1.c	Mathsyaraja H, Catchpole J, Freie B, Eastwood E, Babaeva E, Geuenich M, et al. Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. elife. 2021;10: pubmed publisher
western blot; human; 1:1000; loading ...; fig 4a	Pavlova N, King B, Josselsohn R, Violante S, Macera V, Vardhana S, et al. Translation in amino-acid-poor environments is limited by tRNAGln charging. elife. 2020;9: pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig 6i	Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, et al. UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I. Cell Death Dis. 2020;11:142 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 3e	Liu Z, Mar K, Hanners N, Perelman S, Kanchwala M, Xing C, et al. A NIK-SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-κB. Nature. 2019;: pubmed publisher
chromatin immunoprecipitation; mouse; fig 4b	Garcia Cuellar M, Büttner C, Bartenhagen C, Dugas M, Slany R. Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes. Cell Rep. 2016;15:310-22 pubmed publisher
chromatin immunoprecipitation; human; fig s3	Reeder J, Kwak Y, McNamara R, Forst C, D Orso I. HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells. elife. 2015;4: pubmed publisher
chromatin immunoprecipitation; human	He X, Zhang P. Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair. Mol Cancer. 2015;14:158 pubmed publisher
chromatin immunoprecipitation; rat	Winston J, Li Q, Sarna S. Chronic prenatal stress epigenetically modifies spinal cord BDNF expression to induce sex-specific visceral hypersensitivity in offspring. Neurogastroenterol Motil. 2014;26:715-30 pubmed publisher
chromatin immunoprecipitation; mouse	Li J, Kannan M, Trivett A, Liao H, Wu X, Akagi K, et al. An antisense promoter in mouse L1 retrotransposon open reading frame-1 initiates expression of diverse fusion transcripts and limits retrotransposition. Nucleic Acids Res. 2014;42:4546-62 pubmed publisher
chromatin immunoprecipitation; human; 1:500	Pang C, Toh S, He P, Teissier S, Ben Khalifa Y, Xue Y, et al. A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c). Oncogene. 2014;33:4039-49 pubmed publisher
chromatin immunoprecipitation; chicken	Ellis H, Shioda K, Rosenthal N, Coser K, Shioda T. Masculine epigenetic sex marks of the CYP19A1/aromatase promoter in genetically male chicken embryonic gonads are resistant to estrogen-induced phenotypic sex conversion. Biol Reprod. 2012;87:23, 1-12 pubmed publisher
immunocytochemistry; hamsters western blot; hamsters	Akhrymuk I, Kulemzin S, Frolova E. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol. 2012;86:7180-91 pubmed publisher
chromatin immunoprecipitation; mouse	Weishaupt H, Attema J. A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip. Biol Proced Online. 2010;12:1-17 pubmed publisher
chromatin immunoprecipitation; human; 3 ug	Udelhoven M, Pasieka M, Leeser U, Krone W, Schubert M. Neuronal insulin receptor substrate 2 (IRS2) expression is regulated by ZBP89 and SP1 binding to the IRS2 promoter. J Endocrinol. 2010;204:199-208 pubmed publisher
chromatin immunoprecipitation; human; 3 ug	Udelhoven M, Leeser U, Freude S, Hettich M, Laudes M, Schnitker J, et al. Identification of a region in the human IRS2 promoter essential for stress induced transcription depending on SP1, NFI binding and ERK activation in HepG2 cells. J Mol Endocrinol. 2010;44:99-113 pubmed publisher
chromatin immunoprecipitation; mouse	Leng J, Butcher B, Egan C, Abi Abdallah D, Denkers E. Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation. J Immunol. 2009;182:489-97 pubmed
	Agarwal S, Vierbuchen T, Ghosh S, Chan J, Jiang Z, Kandasamy R, et al. The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans. Nat Commun. 2020;11:6348 pubmed publisher
	Stratton M, Bagchi R, Felisbino M, Hirsch R, Smith H, Riching A, et al. Dynamic Chromatin Targeting of BRD4 Stimulates Cardiac Fibroblast Activation. Circ Res. 2019;125:662-677 pubmed publisher
	Miao H, Wang L, Zhan H, Dai J, Chang Y, Wu F, et al. A long noncoding RNA distributed in both nucleus and cytoplasm operates in the PYCARD-regulated apoptosis by coordinating the epigenetic and translational regulation. PLoS Genet. 2019;15:e1008144 pubmed publisher
	Ogiwara H, Takahashi K, Sasaki M, Kuroda T, Yoshida H, Watanabe R, et al. Targeting the Vulnerability of Glutathione Metabolism in ARID1A-Deficient Cancers. Cancer Cell. 2019;35:177-190.e8 pubmed publisher
	Holmes B, Benavides Serrato A, Freeman R, Landon K, Bashir T, Nishimura R, et al. mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma. Oncogene. 2017;: pubmed publisher
	Ghosh S, Wallerath C, Covarrubias S, Hornung V, Carpenter S, Fitzgerald K. The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression. J Immunol. 2017;199:3249-3260 pubmed publisher
	Schmidt N, Irle I, Ripkens K, Lux V, Nelles J, Johannes C, et al. Epigenetic silencing of serine protease HTRA1 drives polyploidy. BMC Cancer. 2016;16:399 pubmed publisher
	Plummer S, Dan D, Quinney J, Hallmark N, Phillips R, Millar M, et al. Identification of transcription factors and coactivators affected by dibutylphthalate interactions in fetal rat testes. Toxicol Sci. 2013;132:443-57 pubmed publisher
	Woellmer A, Arteaga Salas J, Hammerschmidt W. BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression. PLoS Pathog. 2012;8:e1002902 pubmed publisher
	Song R, Ro S, Michaels J, Park C, McCarrey J, Yan W. Many X-linked microRNAs escape meiotic sex chromosome inactivation. Nat Genet. 2009;41:488-93 pubmed publisher

product information

Catalog Number :

39097

Product Name :

RNA pol II antibody (mAb)

Host Species :

Mouse

Clonality :

Monoclonal

Size :

200 ul

Clone :

4H8

Isotype :

IgG

CrossReactivity :

Budding Yeast, C. elegans, Human, Mouse, Rat

Background :

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Immunogen :

This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.

Uses :

ChIP, ELISA, IF, IP, WB

Storage :

Antibodies in solution can be stored at -800C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.