FAS humanized monoclonal antibody, clone R-125224 | Abnova RAB01165 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Abnova

product type :

antibody

product name :

FAS humanized monoclonal antibody, clone R-125224

catalog :

RAB01165

quantity :

200 ug

clonality :

monoclonal

host :

human

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

RAB01165

Product Name :

FAS humanized monoclonal antibody, clone R-125224

Product Type :

antibody

Host Species :

Human

Clonality :

monoclonal

Conjugation :

Unconjugated

Antigen Modification :

Size :

200 ug

Product Description :

Humanized recombinant monoclonal antibody raised against human R-125224.

Immunogen :

Original antibody is raised against by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fasdeficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas or the L5178YA1 cell line expressing murine Fas, but not to the parental WR19L or L5178Y cell.