gene knockdown

dsRNA Marker

R0005

25 ug

dsRNA Marker

The dsRNA Marker consists of ten double-stranded RNAs, 10, 20, 30, 50, 100, 200, 300, 400, 500 and 1,000 base pairs. The dsRNA Marker is an ideal size marker for determinating sizes of double-stranded RNAs. A twenty bp of RNA band including in the dsRNA Marker is adjusted to approximately 25 ng/ul in concentration. The dsRNA Marker is manufactured for non-denaturing polyacrylamide gel electrophoresis. The dsRNA Marker can be visualized by UV light after ethidium bromide staining.

2 uL/lane

10 mM Tris-HCl (pH 7.5), 50 mM NaCl , 1 mM EDTA

Store at -80 C. Repeated freeze/thaw cycles should be avoided.

After 18 hr incubation of the dsRNA Marker at 37 C, no visible degradation of the marker is observed in 7.5 % polyacrylamide gel electrophoresis.

Even dsRNA is more resistant to RNase than ssRNA, dsRNA is sensitive to degradation by RNase. To avoid damaging the dsRNA Marker, use care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the dsRNA Marker on ice and keep it on ice while using.

DNA/RNA

Electrophoresis

25 ug

10/30/06

8/19/09 0:00