SMAD3 (phospho T179) polyclonal antibody | Abnova PAB19947 product information

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product summary

company name :

Abnova

product type :

antibody

product name :

SMAD3 (phospho T179) polyclonal antibody

catalog :

PAB19947

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

product information

catalog id :

PAB19947

product name :

SMAD3 (phospho T179) polyclonal antibody

product description :

Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.

gene name :

SMAD3

gene alias :

DKFZp586N0721 DKFZp686J10186 HSPC193 HsT17436 JV15-2 MADH3 MGC60396

gene description :

SMAD family member 3

immunogen :

Synthetic phosphopeptide corresponding to residues surrounding T179 of human SMAD3.

form :

Liquid

recommend dilutions :

ELISA (1:15000-1:75000). Western Blot (1:1000). The optimal working dilution should be determined by the end user.

storage buffer :

In 20 mM KH 2 PO 4 , 150 mM NaCl, pH 7.2 (0.01% sodium azide)

storage instruction :

Store at 4 C. For long term storage store at -20 C. Aliquot to avoid repeated freezing and thawing.

note :

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

type clonality :

Antibody

raised in host species :

Rabbit

antigen species target species :

Human

specificity :

This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

species reactivity cross reactivity :

Human

application key :

ELISA,IHC,WB-Ce

size :

100 ug

autodate :

2011-12-02

updatetime :

2015-05-22 19:35:19