Nuclei monoclonal antibody, clone 235-1 | Abnova MAB8178 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Abnova

product type :

antibody

product name :

Nuclei monoclonal antibody, clone 235-1

catalog :

MAB8178

quantity :

100 uL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

235-1

application :

immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

catalog id :

MAB8178

product name :

Nuclei monoclonal antibody, clone 235-1

product description :

Mouse monoclonal antibody raised against native Nuclei.

clone name :

235-1

isotype :

IgG1

immunogen :

Native puridfied from human cell homogenate.

form :

Liquid

recommend dilutions :

Immunofluorescence (1:100). Immunohistochemistry (1:100). The optimal working dilution should be determined by the end user.

storage buffer :

In culture supernatant.

storage instruction :

Store at -20 C. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20 C or -80 C, while keeping a working aliquot stored at 4 C for short term. Aliquot to avoid repeated freezing and thawing.

type clonality :

Antibody

raised in host species :

Mouse

antigen species target species :

Human

specificity :

This antibody reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. Specific for endogenous levels of human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

species reactivity cross reactivity :

Human

application key :

IHC-P,IHC-Fr,IF

size :

100 uL

autodate :

6/1/10

updatetime :

8/27/20 9:17