CD45RA monoclonal antibody, clone PTPRC/1148 | Abnova MAB13026 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Abnova

product type :

antibody

product name :

CD45RA monoclonal antibody, clone PTPRC/1148

catalog :

MAB13026

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PTPRC/1148

The same clone is also sold as:

Abcam: ab218501, ab212774

reactivity :

human

application :

immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

catalog id :

MAB13026

product name :

CD45RA monoclonal antibody, clone PTPRC/1148

product description :

Mouse monoclonal antibody raised against full length recombinant human CD45RA.

clone name :

PTPRC/1148

isotype :

IgG1, kappa

gene name :

PTPRC

gene alias :

B220 CD45 CD45R GP180 LCA LY5 T200

gene description :

protein tyrosine phosphatase, receptor type, C

immunogen :

Recombinant protein corresponding to full length of human CD45RA.

form :

Liquid

recommend dilutions :

Flow Cytometry (0.5-1 ug/million cells in 0.1 mL). Immunofluorescence (1-2 ug/mL). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL). The optimal working dilution should be determined by the end user.

storage buffer :

In 10 mM PBS (0.05% BSA, 0.05% sodium azide).

storage instruction :

Store at 4 C.

note :

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

type clonality :

Antibody

raised in host species :

Mouse

antigen species target species :

Human

specificity :

Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

species reactivity cross reactivity :

Human

application key :

IHC-P,IF,Flow Cyt

size :

100 ug

autodate :

2016-02-26

updatetime :

2016-02-26 16:58:45