GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

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product summary

company name :

Abnova

product type :

other

product name :

GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

catalog :

L100T6

quantity :

100 ug

product information

catalog id :

L100T6

product name :

GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

gene name :

GBA

gene alias :

GBA1 GCB GLUC

gene description :

glucosidase, beta; acid (includes glucosylceramidase)

genbank accession :

NM_000157

protein accession :

NP_000148

concentration :

2 mg/ml

plasmid :

pCMV-GBA full length

lysis buffer :

Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

host cell :

HEK293

recommend dilutions :

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

storage buffer :

In modified RIPA Lysis Buffer.

storage instruction :

Store at -80 C. Aliquot to avoid repeated freezing and thawing.

quality control testing :

Transient overexpression cell lysate was tested with Anti-GBA antibody ( H00002629-M01 ) by Western Blots.

type clonality :

Lysate

raised in host species :

Human

antigen species target species :

Human

application key :

WB,IP

size :

100 ug

autodate :

2007-04-12

updatetime :

2011-05-18 11:19:39