other

Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)

L024V1

200 ug

Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) whole cell lysate (denatured)

Whole cell lysate (denatured)

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Macrophage

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95 C for 5 min, and cooled rapidly.

The lysate is ready to load on SDS-PAGE for Western blotting.

In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).

Store at -80 C. Aliquot to avoid repeated freezing and thawing.

12.5% SDS-PAGE Stained with Coomassie Blue.

Lysate

Mouse

Mouse

WB

200 ug

2006-10-10

2014-11-18 13:56:05