HeLa (human cervical epithelioid carcinoma) whole cell lysate (non-denatured)

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product summary

company name :

Abnova

product type :

other

product name :

HeLa (human cervical epithelioid carcinoma) whole cell lysate (non-denatured)

catalog :

L013V2

quantity :

200 ug

product information

catalog id :

L013V2

product name :

HeLa (human cervical epithelioid carcinoma) whole cell lysate (non-denatured)

product description :

Whole cell lysate (non-denatured)

lysis buffer :

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

tissue :

Cervix

preparation method :

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

recommend dilutions :

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

storage buffer :

In modified RIPA Lysis Buffer.

storage instruction :

Store at -80 C. Aliquot to avoid repeated freezing and thawing.

quality control testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

type clonality :

Lysate

raised in host species :

Human

antigen species target species :

Human

application key :

IP,WB

size :

200 ug

autodate :

11/30/06

updatetime :

11/18/14 13:56