Hela (human cervical epithelioid carcinoma) whole cell lysate (denatured)

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product summary

company name :

Abnova

product type :

other

product name :

Hela (human cervical epithelioid carcinoma) whole cell lysate (denatured)

catalog :

L013V1

quantity :

200 ug

product information

catalog id :

L013V1

product name :

Hela (human cervical epithelioid carcinoma) whole cell lysate (denatured)

product description :

Whole cell lysate (denatured)

lysis buffer :

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

tissue :

Cervix

preparation method :

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95 C for 5 min, and cooled rapidly.

recommend dilutions :

The lysate is ready to load on SDS-PAGE for Western blotting.

storage buffer :

In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).

storage instruction :

Store at -80 C. Aliquot to avoid repeated freezing and thawing.

quality control testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

type clonality :

Lysate

raised in host species :

Human

antigen species target species :

Human

application key :

size :

200 ug

autodate :

2006-10-10

updatetime :

2014-11-18 13:56:05