KRAS monoclonal antibody (M01), clone 3B10-2F2 | Abnova H00003845-M01 product information

product summary

company name :

Abnova

product type :

antibody

product name :

KRAS monoclonal antibody (M01), clone 3B10-2F2

catalog :

H00003845-M01

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B10-2F2

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Abnova product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human	Amen A, Loughran R, Huang C, Lew R, Ravi A, Guan Y, et al. Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi. Cell Rep Methods. 2022;2:100239 pubmed publisher
western blot; mouse; loading ...; fig 3e western blot; human; loading ...; fig 3b; 4a	Forzati F, De Martino M, Esposito F, Sepe R, Pellecchia S, Malapelle U, et al. miR-155 is positively regulated by CBX7 in mouse embryonic fibroblasts and colon carcinomas, and targets the KRAS oncogene. BMC Cancer. 2017;17:170 pubmed publisher
western blot; human; loading ...; fig s6	Madureira P, Bharadwaj A, Bydoun M, Garant K, O Connell P, Lee P, et al. Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10. Oncotarget. 2016;7:47720-47737 pubmed publisher
immunoprecipitation; mouse immunohistochemistry; mouse; 1:50 western blot; mouse; 1:2000	Krossa S, Schmitt A, Hattermann K, Fritsch J, Scheidig A, Mehdorn H, et al. Down regulation of Akirin-2 increases chemosensitivity in human glioblastomas more efficiently than Twist-1. Oncotarget. 2015;6:21029-45 pubmed
	Cao S, Chung S, Kim S, Li Z, Manor D, Buck M. K-Ras G-domain binding with signaling lipid phosphatidylinositol (4,5)-phosphate (PIP2): membrane association, protein orientation, and function. J Biol Chem. 2019;294:7068-7084 pubmed publisher
	Valtorta E, Misale S, Sartore Bianchi A, Nagtegaal I, Paraf F, Lauricella C, et al. KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy. Int J Cancer. 2013;133:1259-65 pubmed publisher
	Fuentes Calvo I, Blázquez Medela A, Santos E, Lopez Novoa J, Martinez Salgado C. Analysis of k-ras nuclear expression in fibroblasts and mesangial cells. PLoS ONE. 2010;5:e8703 pubmed publisher

product information

catalog id :

H00003845-M01

product name :

KRAS monoclonal antibody (M01), clone 3B10-2F2

product description :

Mouse monoclonal antibody raised against a full length recombinant KRAS.

clone name :

3B10-2F2

isotype :

IgG1 Kappa

gene name :

KRAS

gene alias :

C-K-RAS K-RAS2A K-RAS2B K-RAS4A K-RAS4B KI-RAS KRAS1 KRAS2 NS3 RASK2

gene description :

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

genbank accession :

BC013572

immunogen :

KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

immunogen sequence protein sequence :

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDS
YRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGF
LCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNK
CDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAF
YTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

protein accession :

AAH13572

storage buffer :

In 1x PBS, pH 7.4

storage instruction :

Store at -20 C or lower. Aliquot to avoid repeated freezing and thawing

quality control testing :

Antibody Reactive Against Recombinant Protein

type clonality :

Antibody

raised in host species :

Mouse

antigen species target species :

Human

species reactivity cross reactivity :

Human

application key :

WB-Tr,S-ELISA,ELISA,WB-Re,WB-Ce,IF

size :

100 ug

autodate :

4/30/08

updatetime :

11/15/13 18:40

more info or order :

Abnova product webpage