product summary
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company name :
Abnova
product type :
antibody
product name :
H3F3B monoclonal antibody (M01), clone 2D7-H1
catalog :
H00003021-M01
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D7-H1
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation
more info or order :
citations: 6
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:40; fig 4
Jang C, Shibata Y, Starmer J, Yee D, Magnuson T. Histone H3.3 maintains genome integrity during mammalian development. Genes Dev. 2015;29:1377-92 pubmed publisher
Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, et al. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun. 2020;11:913 pubmed publisher
Chronis C, Fiziev P, Papp B, Butz S, Bonora G, Sabri S, et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming. Cell. 2017;168:442-459.e20 pubmed publisher
Lin C, Conti M, Ramalho Santos M. Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development. Development. 2013;140:3624-34 pubmed publisher
Jullien J, Astrand C, Szenker E, Garrett N, Almouzni G, Gurdon J. HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes. Epigenetics Chromatin. 2012;5:17 pubmed publisher
Drané P, Ouararhni K, Depaux A, Shuaib M, Hamiche A. The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3. Genes Dev. 2010;24:1253-65 pubmed publisher
product information
catalog id :
H00003021-M01
product name :
H3F3B monoclonal antibody (M01), clone 2D7-H1
product description :
Mouse monoclonal antibody raised against a full length recombinant H3F3B.
clone name :
2D7-H1
isotype :
IgG1 kappa
gene name :
H3F3B
gene alias :
H3.3B H3F3A
gene description :
H3 histone, family 3B (H3.3B)
genbank accession :
BC017558
immunogen :
H3F3B (AAH17558, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
immunogen sequence protein sequence :
MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKP
HRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQD
FKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKR
VTIMPKDIQLARRIRGERA
protein accession :
AAH17558
storage buffer :
In 1x PBS, pH 7.4
storage instruction :
Store at -20 C or lower. Aliquot to avoid repeated freezing and thawing
quality control testing :
Antibody Reactive Against Recombinant Protein
type clonality :
Antibody
raised in host species :
Mouse
antigen species target species :
Human
species reactivity cross reactivity :
Human
application key :
S-ELISA,IF,ELISA,WB-Re,WB-Tr,IHC-P
size :
100 ug
autodate :
10/10/06
updatetime :
11/15/13 18:29
more info or order :
company information
Abnova
9th Fl., No.108, Jhouzih St.
Neihu District. Taipei City
114 Taiwan
sales@abnova.com
https://www.abnova.com
877-853-6098