product summary
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company name :
Abnova
product type :
antibody
product name :
ATP6V1A monoclonal antibody (M02), clone 4F5
catalog :
H00000523-M02
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4F5
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 2e
  • immunocytochemistry; human; 1:250; loading ...; fig 2a, 2d
  • western blot; human; 1:1000; loading ...; fig e1c, e1g
Ramírez C, Hauser A, Vucic E, Bar Sagi D. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature. 2019;576:477-481 pubmed publisher
  • western blot; human; fig 2
Smith G, Howell G, Phillips C, Muench S, Ponnambalam S, Harrison M. Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes. J Biol Chem. 2016;291:8500-15 pubmed publisher
product information
catalog id :
H00000523-M02
product name :
ATP6V1A monoclonal antibody (M02), clone 4F5
product description :
Mouse monoclonal antibody raised against a partial recombinant ATP6V1A.
clone name :
4F5
isotype :
IgG1 Kappa
gene name :
ATP6V1A
gene alias :
ATP6A1 ATP6V1A1 HO68 VA68 VPP2 Vma1
gene description :
ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
genbank accession :
NM_001690
immunogen :
ATP6V1A (NP_001681, 508 a.a. ~ 617 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
immunogen sequence protein sequence :
TLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIA
FYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMK
FKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED
protein accession :
NP_001681
storage buffer :
In 1x PBS, pH 7.4
storage instruction :
Store at -20 C or lower. Aliquot to avoid repeated freezing and thawing
quality control testing :
Antibody Reactive Against Recombinant Protein
type clonality :
Antibody
raised in host species :
Mouse
antigen species target species :
Human
species reactivity cross reactivity :
Human
application key :
IF,WB-Tr,S-ELISA,WB-Ti,IHC-P,ELISA,WB-Re
size :
100 ug
autodate :
6/15/09
updatetime :
11/15/13 18:43
more info or order :
company information
Abnova
9th Fl., No.108, Jhouzih St.
Neihu District. Taipei City
114 Taiwan
sales@abnova.com
https://www.abnova.com
877-853-6098