This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Ascent Scientific
product type :
antibody
product name :
NFkB p65 antibody - ChIP Grade
catalog :
ab7970
quantity :
1 ml
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 131
Published Application/Species/DilutionReference
  • chromatin immunoprecipitation; human; fig 5
Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Shimizu K, Tachibana M, et al. NF-κB promotes leaky expression of adenovirus genes in a replication-incompetent adenovirus vector. Sci Rep. 2016;6:19922 pubmed publisher
  • immunohistochemistry; human; 1:1000; fig s5
Huang Q, Zhan L, Cao H, Li J, Lyu Y, Guo X, et al. Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. Autophagy. 2016;12:999-1014 pubmed publisher
  • western blot; rat; fig 4
  • immunohistochemistry; rat; 1:200; fig 3
Garcia P, Seiva F, Carniato A, de Mello Júnior W, Duran N, Macedo A, et al. Increased toll-like receptors and p53 levels regulate apoptosis and angiogenesis in non-muscle invasive bladder cancer: mechanism of action of P-MAPA biological response modifier. BMC Cancer. 2016;16:422 pubmed publisher
  • western blot; pig; 1:5000; fig 2
Ganesan S, Reynolds C, Hollinger K, Pearce S, Gabler N, Baumgard L, et al. Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2016;310:R1288-96 pubmed publisher
  • western blot; mouse; fig 6
Li J, Hardy K, Phetsouphanh C, Tu W, Sutcliffe E, McCuaig R, et al. Nuclear PKC-θ facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci. 2016;129:2448-61 pubmed publisher
  • chromatin immunoprecipitation; human; fig 2
Garcia V, Shkolnik B, Milhau L, Falck J, Schwartzman M. 20-HETE Activates the Transcription of Angiotensin-Converting Enzyme via Nuclear Factor-κB Translocation and Promoter Binding. J Pharmacol Exp Ther. 2016;356:525-33 pubmed publisher
  • western blot; human; fig 1
  • western blot; mouse; fig 1
West A, Martin B, Andrews D, Hogg S, Banerjee A, Grigoriadis G, et al. The SMAC mimetic, LCL-161, reduces survival in aggressive MYC-driven lymphoma while promoting susceptibility to endotoxic shock. Oncogenesis. 2016;5:e216 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 9
Kropp K, Hsieh W, Isern E, Forster T, Krause E, Brune W, et al. A temporal gate for viral enhancers to co-opt Toll-like-receptor transcriptional activation pathways upon acute infection. PLoS Pathog. 2015;11:e1004737 pubmed publisher
  • western blot; rat
Bharti S, Rani N, Bhatia J, Arya D. 5-HT2B receptor blockade attenuates β-adrenergic receptor-stimulated myocardial remodeling in rats via inhibiting apoptosis: role of MAPKs and HSPs. Apoptosis. 2015;20:455-65 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 5
Lu W, Bird T, Boulter L, Tsuchiya A, Cole A, Hay T, et al. Hepatic progenitor cells of biliary origin with liver repopulation capacity. Nat Cell Biol. 2015;17:971-83 pubmed publisher
  • western blot; human; fig 6
Yang Y, Kim D, Seo Y, Park D, Jang H, Choi S, et al. Elevated O-GlcNAcylation promotes colonic inflammation and tumorigenesis by modulating NF-κB signaling. Oncotarget. 2015;6:12529-42 pubmed
  • western blot; mouse; fig 2
Liu Q, Zhu Y, Yong W, Sze N, Tan N, Ding J. Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response. J Immunol. 2015;195:801-5 pubmed publisher
  • western blot; rat
Garcia P, Apolinário L, Böckelmann P, da Silva Nunes I, Duran N, Fávaro W. Alterations in ubiquitin ligase Siah-2 and its corepressor N-CoR after P-MAPA immunotherapy and anti-androgen therapy: new therapeutic opportunities for non-muscle invasive bladder cancer. Int J Clin Exp Pathol. 2015;8:4427-43 pubmed
  • chromatin immunoprecipitation; mouse
Collier S, Henderson M, Tossberg J, Aune T. Regulation of the Th1 genomic locus from Ifng through Tmevpg1 by T-bet. J Immunol. 2014;193:3959-65 pubmed publisher
  • chromatin immunoprecipitation; mouse; 3.5 ug
Stilling R, Rönicke R, Benito E, Urbanke H, Capece V, Burkhardt S, et al. K-Lysine acetyltransferase 2a regulates a hippocampal gene expression network linked to memory formation. EMBO J. 2014;33:1912-27 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200
Zhao L, Song Y, Pu J, Guo J, Wang Y, Chen Z, et al. Effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress. Toxicol Lett. 2014;231:72-81 pubmed publisher
  • western blot; rat; 1:1000
Bao Y, Hou W, Liu R, Gao Y, Kong X, Yang L, et al. PAR2-mediated upregulation of BDNF contributes to central sensitization in bone cancer pain. Mol Pain. 2014;10:28 pubmed publisher
  • immunohistochemistry; rat; 1:100
Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, et al. Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. PLoS ONE. 2014;9:e106331 pubmed publisher
  • western blot; human
Cumming K, Raastad T, Holden G, Bastani N, Schneeberger D, Paronetto M, et al. Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training. Physiol Rep. 2014;2: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, et al. B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling. PLoS ONE. 2014;9:e91373 pubmed publisher
  • EMSA; human
  • chromatin immunoprecipitation; human
Datta De D, Datta A, Bhattacharjya S, Roychoudhury S. NF-kappaB mediated transcriptional repression of acid modifying hormone gastrin. PLoS ONE. 2013;8:e73409 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000
Domitrovic R, Cvijanovic O, Pernjak-Pugel E, Skoda M, Mikelić L, Crncevic-Orlic Z. Berberine exerts nephroprotective effect against cisplatin-induced kidney damage through inhibition of oxidative/nitrosative stress, inflammation, autophagy and apoptosis. Food Chem Toxicol. 2013;62:397-406 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Domitrovic R, Cvijanovic O, Pugel E, Zagorac G, Mahmutefendić H, Skoda M. Luteolin ameliorates cisplatin-induced nephrotoxicity in mice through inhibition of platinum accumulation, inflammation and apoptosis in the kidney. Toxicology. 2013;310:115-23 pubmed publisher
Zavasnik-Bergant T, Bergant Marušič M. Exogenous Thyropin from p41 Invariant Chain Diminishes Cysteine Protease Activity and Affects IL-12 Secretion during Maturation of Human Dendritic Cells. PLoS ONE. 2016;11:e0150815 pubmed publisher
Akdogan R, Kalkan Y, Tumkaya L, Rakıcı H, Akdogan E. The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue. Gastroenterol Res Pract. 2016;2016:1738430 pubmed publisher
Porreca I, Ulloa Severino L, D'Angelo F, Cuomo D, Ceccarelli M, Altucci L, et al. "Stockpile" of Slight Transcriptomic Changes Determines the Indirect Genotoxicity of Low-Dose BPA in Thyroid Cells. PLoS ONE. 2016;11:e0151618 pubmed publisher
Guo J, Chen L, Luo N, Li C, Chen R, Qu X, et al. LPS/TLR4-mediated stromal cells acquire an invasive phenotype and are implicated in the pathogenesis of adenomyosis. Sci Rep. 2016;6:21416 pubmed publisher
Shatz M, Shats I, Menendez D, Resnick M. p53 amplifies Toll-like receptor 5 response in human primary and cancer cells through interaction with multiple signal transduction pathways. Oncotarget. 2015;6:16963-80 pubmed
Hu J, Meng Y, Yu T, Hu L, Mao M. Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth. Oncotarget. 2015;6:12174-87 pubmed
Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, et al. Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer. BMC Cancer. 2015;15:34 pubmed publisher
Komaravelli N, Kelley J, Garofalo M, Wu H, Casola A, Kolli D. Role of dietary antioxidants in human metapneumovirus infection. Virus Res. 2015;200:19-23 pubmed publisher
Wilson C, Jurk D, Fullard N, Banks P, Page A, Luli S, et al. NFκB1 is a suppressor of neutrophil-driven hepatocellular carcinoma. Nat Commun. 2015;6:6818 pubmed publisher
Mastropietro G, Tiscornia I, Perelmuter K, Astrada S, Bollati-Fogolin M. HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation. Mediators Inflamm. 2015;2015:860534 pubmed publisher
Yang Z, Fang S, Di Y, Ying W, Tan Y, Gu W. Modulation of NF-κB/miR-21/PTEN pathway sensitizes non-small cell lung cancer to cisplatin. PLoS ONE. 2015;10:e0121547 pubmed publisher
Wu M, Chen J, Wang Y, Hu J, Liu C, Feng C, et al. URGCP/URG4 promotes apoptotic resistance in bladder cancer cells by activating NF-κB signaling. Oncotarget. 2015;6:30887-901 pubmed publisher
Wang L, Song G, Zheng Y, Tan W, Pan J, Zhao Y, et al. Expression of Semaphorin 4A and its potential role in rheumatoid arthritis. Arthritis Res Ther. 2015;17:227 pubmed publisher
Mikula M, Majewska A, Ledwon J, Dzwonek A, Ostrowski J. Obesity increases histone H3 lysine 9 and 18 acetylation at Tnfa and Ccl2 genes in mouse liver. Int J Mol Med. 2014;34:1647-54 pubmed publisher
Hyun J, Choi S, Diehl A, Jung Y. Potential role of Hedgehog signaling and microRNA-29 in liver fibrosis of IKKβ-deficient mouse. J Mol Histol. 2014;45:103-12 pubmed publisher
Spurlock C, Tossberg J, Matlock B, Olsen N, Aune T. Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction. Arthritis Rheumatol. 2014;66:2947-57 pubmed publisher
Wann A, Chapple J, Knight M. The primary cilium influences interleukin-1β-induced NFκB signalling by regulating IKK activity. Cell Signal. 2014;26:1735-42 pubmed publisher
Kuroda N, Inoue K, Ikeda T, Hara Y, Wake K, Sato T. Apoptotic response through a high mobility box 1 protein-dependent mechanism in LPS/GalN-induced mouse liver failure and glycyrrhizin-mediated inhibition. PLoS ONE. 2014;9:e92884 pubmed publisher
Xu H, Lai W, Zhang Y, Liu L, Luo X, Zeng Y, et al. Tumor-associated macrophage-derived IL-6 and IL-8 enhance invasive activity of LoVo cells induced by PRL-3 in a KCNN4 channel-dependent manner. BMC Cancer. 2014;14:330 pubmed publisher
Dang Y, Xu Y, Wu W, Li W, Sun Y, Yang J, et al. Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells. PLoS ONE. 2014;9:e102522 pubmed publisher
Johnson J, Jenkins N, Huang W, Di Gregoli K, Sala-Newby G, Scholtes V, et al. Relationship of MMP-14 and TIMP-3 expression with macrophage activation and human atherosclerotic plaque vulnerability. Mediators Inflamm. 2014;2014:276457 pubmed publisher
Du J, An R, Chen L, Shen Y, Chen Y, Cheng L, et al. Toxoplasma gondii virulence factor ROP18 inhibits the host NF-κB pathway by promoting p65 degradation. J Biol Chem. 2014;289:12578-92 pubmed publisher
Canello T, Ovadia H, Refael M, Zrihan D, Siegal T, Lavon I. Antineoplastic effect of decoy oligonucleotide derived from MGMT enhancer. PLoS ONE. 2014;9:e113854 pubmed publisher
Luo X, Chae M, Krishnakumar R, Danko C, Kraus W. Dynamic reorganization of the AC16 cardiomyocyte transcriptome in response to TNFα signaling revealed by integrated genomic analyses. BMC Genomics. 2014;15:155 pubmed publisher
Zhang B, Zhou M, Li C, Zhou J, Li H, Zhu D, et al. MicroRNA-92a inhibition attenuates hypoxia/reoxygenation-induced myocardiocyte apoptosis by targeting Smad7. PLoS ONE. 2014;9:e100298 pubmed publisher
Foo J, Yazan L, Tor Y, Armania N, Ismail N, Imam M, et al. Induction of cell cycle arrest and apoptosis in caspase-3 deficient MCF-7 cells by Dillenia suffruticosa root extract via multiple signalling pathways. BMC Complement Altern Med. 2014;14:197 pubmed publisher
Krawczyk M, Emerson B. p50-associated COX-2 extragenic RNA (PACER) activates COX-2 gene expression by occluding repressive NF-κB complexes. elife. 2014;3:e01776 pubmed publisher
Cheng Z, Li-Sha G, Jing-Lin Z, Wen-Wu Z, Xue-Si C, Xing-Xing C, et al. Protective role of the cholinergic anti-inflammatory pathway in a mouse model of viral myocarditis. PLoS ONE. 2014;9:e112719 pubmed publisher
DeDiego M, Nieto-Torres J, Regla-Nava J, Jiménez-Guardeño J, Fernández-Delgado R, Fett C, et al. Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival. J Virol. 2014;88:913-24 pubmed publisher
Meziani F, Kremer H, Tesse A, Baron-Menguy C, Mathien C, Mostefai H, et al. Human serum albumin improves arterial dysfunction during early resuscitation in mouse endotoxic model via reduced oxidative and nitrosative stresses. Am J Pathol. 2007;171:1753-61 pubmed
Yen H, Ooka T, Iguchi A, Hayashi T, Sugimoto N, Tobe T. NleC, a type III secretion protease, compromises NF-κB activation by targeting p65/RelA. PLoS Pathog. 2010;6:e1001231 pubmed publisher
Tesse A, Al-Massarani G, Wangensteen R, Reitenbach S, Martinez M, Andriantsitohaina R. Rosiglitazone, a peroxisome proliferator-activated receptor-gamma agonist, prevents microparticle-induced vascular hyporeactivity through the regulation of proinflammatory proteins. J Pharmacol Exp Ther. 2008;324:539-47 pubmed
Danzer E, Kiddoo D, Redden R, Robinson L, Radu A, Zderic S, et al. Structural and functional characterization of bladder smooth muscle in fetal rats with retinoic acid-induced myelomeningocele. Am J Physiol Renal Physiol. 2007;292:F197-206 pubmed
Dryden N, Sperone A, Martin-Almedina S, Hannah R, Birdsey G, Khan S, et al. The transcription factor Erg controls endothelial cell quiescence by repressing activity of nuclear factor (NF)-κB p65. J Biol Chem. 2012;287:12331-42 pubmed publisher
So J, Kwon H, Lee C, Yi H, Park J, Lim S, et al. Lactobacillus casei suppresses experimental arthritis by down-regulating T helper 1 effector functions. Mol Immunol. 2008;45:2690-9 pubmed publisher
Rapicavoli N, Qu K, Zhang J, Mikhail M, Laberge R, Chang H. A mammalian pseudogene lncRNA at the interface of inflammation and anti-inflammatory therapeutics. elife. 2013;2:e00762 pubmed publisher
Gao X, Zhang H, Schmidt A, Zhang C. AGE/RAGE produces endothelial dysfunction in coronary arterioles in type 2 diabetic mice. Am J Physiol Heart Circ Physiol. 2008;295:H491-8 pubmed publisher
Chen Y, Chiang S, Wu H, Kao S, Hsiang C, Ho T, et al. Microarray analysis reveals the inhibition of nuclear factor-kappa B signaling by aristolochic acid in normal human kidney (HK-2) cells. Acta Pharmacol Sin. 2010;31:227-36 pubmed publisher
Leu J, Murphy M, George D. The p53 Codon 72 Polymorphism Modifies the Cellular Response to Inflammatory Challenge in the Liver. J Liver. 2013;2: pubmed
Bhoopathi P, Chetty C, Kunigal S, Vanamala S, Rao J, Lakka S. Blockade of tumor growth due to matrix metalloproteinase-9 inhibition is mediated by sequential activation of beta1-integrin, ERK, and NF-kappaB. J Biol Chem. 2008;283:1545-52 pubmed
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Du N, Feng J, Hu L, Sun X, Sun H, Zhao Y, et al. Angiotensin II receptor type 1 blockers suppress the cell proliferation effects of angiotensin II in breast cancer cells by inhibiting AT1R signaling. Oncol Rep. 2012;27:1893-903 pubmed publisher
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Harikumar K, Sung B, Tharakan S, Pandey M, Joy B, Guha S, et al. Sesamin manifests chemopreventive effects through the suppression of NF-kappa B-regulated cell survival, proliferation, invasion, and angiogenic gene products. Mol Cancer Res. 2010;8:751-61 pubmed publisher
Kovacic J, Gupta R, Lee A, Ma M, Fang F, Tolbert C, et al. Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice. J Clin Invest. 2010;120:303-14 pubmed publisher
Tichelaar J, Zhang Y, leRiche J, Biddinger P, Lam S, Anderson M. Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies. BMC Cancer. 2005;5:155 pubmed
Sanchez A, Tripathy D, Yin X, Luo J, Martinez J, Grammas P. Sunitinib enhances neuronal survival in vitro via NF-κB-mediated signaling and expression of cyclooxygenase-2 and inducible nitric oxide synthase. J Neuroinflammation. 2013;10:93 pubmed publisher
Xiao H, Gulen M, Qin J, Yao J, Bulek K, Kish D, et al. The Toll-interleukin-1 receptor member SIGIRR regulates colonic epithelial homeostasis, inflammation, and tumorigenesis. Immunity. 2007;26:461-75 pubmed
Lin J, Guan Z, Wang C, Feng L, Zheng Y, Caicedo E, et al. Inhibitor of differentiation 1 contributes to head and neck squamous cell carcinoma survival via the NF-kappaB/survivin and phosphoinositide 3-kinase/Akt signaling pathways. Clin Cancer Res. 2010;16:77-87 pubmed publisher
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Jani T, DeVecchio J, Mazumdar T, Agyeman A, Houghton J. Inhibition of NF-kappaB signaling by quinacrine is cytotoxic to human colon carcinoma cell lines and is synergistic in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) or oxaliplatin. J Biol Chem. 2010;285:19162-72 pubmed publisher
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Howe E, Cochrane D, Cittelly D, Richer J. miR-200c targets a NF-κB up-regulated TrkB/NTF3 autocrine signaling loop to enhance anoikis sensitivity in triple negative breast cancer. PLoS ONE. 2012;7:e49987 pubmed publisher
Gunella G, Bardelli C, Amoruso A, Viano I, Balbo P, Brunelleschi S. Macrophage-stimulating protein differently affects human alveolar macrophages from smoker and non-smoker patients: evaluation of respiratory burst, cytokine release and NF-kappaB pathway. Br J Pharmacol. 2006;148:478-89 pubmed
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Nozell S, Laver T, Patel K, Benveniste E. Mechanism of IFN-beta-mediated inhibition of IL-8 gene expression in astroglioma cells. J Immunol. 2006;177:822-30 pubmed
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Abdullah M, Rani A, Sudoyo A, Makmun D, Handjari D, Hernowo B. Expression of NF-kB and COX2 in colorectal cancer among native Indonesians: the role of inflammation in colorectal carcinogenesis. Acta Med Indones. 2013;45:187-92 pubmed
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Whitham M, Chan M, Pal M, Matthews V, Prelovsek O, Lunke S, et al. Contraction-induced interleukin-6 gene transcription in skeletal muscle is regulated by c-Jun terminal kinase/activator protein-1. J Biol Chem. 2012;287:10771-9 pubmed publisher
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product information
Catalog Number :
ab7970
Product Name :
NFkB p65 antibody - ChIP Grade
Host Species :
Rabbit
Clonality :
polyclonal
Product Type :
Primary antibodies
Size :
1 ml
SwissProtID :
Q04206
Isotype :
IgG
CrossReactivity :
Reacts with: Human, Mouse, Rat.
Application Summary :
ChIP, ChIP/Chip, EMSA, Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB
company information
Abcam
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USA
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