This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Ascent Scientific
product type :
antibody
product name :
p21 antibody
catalog :
ab7960
quantity :
1 ml
clonality :
polyclonal
host :
rabbit
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 66
Published Application/Species/DilutionReference
  • immunohistochemistry; mouse; 1:500; fig 3
Cacabelos D, Ramírez-Nuñez O, Granado-Serrano A, Torres P, Ayala V, Moiseeva V, et al. Early and gender-specific differences in spinal cord mitochondrial function and oxidative stress markers in a mouse model of ALS. Acta Neuropathol Commun. 2016;4:3 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Li X, Cheng K, Liu Z, Yang J, Wang B, Jiang X, et al. The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells. Nat Commun. 2016;7:11740 pubmed publisher
  • western blot; mouse; 1:1000; fig s1
Safdar A, Khrapko K, Flynn J, Saleem A, De Lisio M, Johnston A, et al. Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice. Skelet Muscle. 2016;6:7 pubmed publisher
  • western blot; human; 1:1000; fig 1a
  • western blot; mouse; fig 1c
Katlinskaya Y, Katlinski K, Yu Q, Ortiz A, Beiting D, Brice A, et al. Suppression of Type I Interferon Signaling Overcomes Oncogene-Induced Senescence and Mediates Melanoma Development and Progression. Cell Rep. 2016;15:171-80 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 1
  • western blot; human; 1:200; fig 2s1
Fong C, Mazo G, Das T, Goodman J, Kim M, O'Rourke B, et al. 53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis. elife. 2016;5: pubmed publisher
  • immunocytochemistry; human; 1:100
  • western blot; human
Kim S, Lahmy R, Riha C, Yang C, Jakubison B, van Niekerk J, et al. The basic helix-loop-helix transcription factor E47 reprograms human pancreatic cancer cells to a quiescent acinar state with reduced tumorigenic potential. Pancreas. 2015;44:718-27 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 4
Birch J, Anderson R, Correia-Melo C, Jurk D, Hewitt G, Marques F, et al. DNA damage response at telomeres contributes to lung aging and chronic obstructive pulmonary disease. Am J Physiol Lung Cell Mol Physiol. 2015;309:L1124-37 pubmed publisher
  • western blot; human; fig 4
Doldi V, Callari M, Giannoni E, D'Aiuto F, Maffezzini M, Valdagni R, et al. Integrated gene and miRNA expression analysis of prostate cancer associated fibroblasts supports a prominent role for interleukin-6 in fibroblast activation. Oncotarget. 2015;6:31441-60 pubmed publisher
  • western blot; human; 1:1000
Shoji K, Teishima J, Hayashi T, Ohara S, McKeehan W, Matsubara A. Restoration of fibroblast growth factor receptor 2IIIb enhances the chemosensitivity of human prostate cancer cells. Oncol Rep. 2014;32:65-70 pubmed publisher
  • western blot; human
  • immunohistochemistry; human
Huang Y, Chen J, Lu C, Han J, Wang G, Song C, et al. HDAC1 and Klf4 interplay critically regulates human myeloid leukemia cell proliferation. Cell Death Dis. 2014;5:e1491 pubmed publisher
  • western blot; mouse
Brown D, LASSEGUE B, Lee M, Zafari R, Long J, Saavedra H, et al. Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts. PLoS ONE. 2014;9:e96657 pubmed publisher
  • western blot; mouse; 1:1000
Ma X, Choudhury S, Hua X, Dai Z, Li Y. Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway. Carcinogenesis. 2013;34:1216-23 pubmed publisher
  • western blot; mouse
Singh K, Shukla P, Quan A, Desjardins J, Lovren F, Pan Y, et al. BRCA2 protein deficiency exaggerates doxorubicin-induced cardiomyocyte apoptosis and cardiac failure. J Biol Chem. 2012;287:6604-14 pubmed publisher
  • western blot; mouse; fig s5a
Joaquin M, Gubern A, Gonzalez-Nunez D, Josué Ruiz E, Ferreiro I, de Nadal E, et al. The p57 CDKi integrates stress signals into cell-cycle progression to promote cell survival upon stress. EMBO J. 2012;31:2952-64 pubmed publisher
Nelson D, Jaber-Hijazi F, Cole J, Robertson N, Pawlikowski J, Norris K, et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 2016;17:158 pubmed publisher
Goto Y, Yajima I, Kumasaka M, Ohgami N, Tanaka A, Tsuzuki T, et al. Transcription factor LSF (TFCP2) inhibits melanoma growth. Oncotarget. 2016;7:2379-90 pubmed publisher
Zhang Y, Zhang G, Li X, Li B, Zhang X. The effect of ribosomal protein S15a in lung adenocarcinoma. Peerj. 2016;4:e1792 pubmed publisher
Rabinovich R, Drost E, Manning J, Dunbar D, Díaz-Ramos M, Lakhdar R, et al. Genome-wide mRNA expression profiling in vastus lateralis of COPD patients with low and normal fat free mass index and healthy controls. Respir Res. 2015;16:1 pubmed publisher
Grootaert M, da Costa Martins P, Bitsch N, Pintelon I, De Meyer G, Martinet W, et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy. 2015;11:2014-2032 pubmed
Minieri V, Saviozzi S, Gambarotta G, Lo Iacono M, Accomasso L, Cibrario Rocchietti E, et al. Persistent DNA damage-induced premature senescence alters the functional features of human bone marrow mesenchymal stem cells. J Cell Mol Med. 2015;19:734-43 pubmed publisher
Pan C, Lin W, Chien Y, Liu F, Sheu M, Kuo Y, et al. K20E, an oxidative-coupling compound of methyl caffeate, exhibits anti-angiogenic activities through down-regulations of VEGF and VEGF receptor-2. Toxicol Appl Pharmacol. 2015;282:215-26 pubmed publisher
Cosgrove B, Gilbert P, Porpiglia E, Mourkioti F, Lee S, Corbel S, et al. Rejuvenation of the muscle stem cell population restores strength to injured aged muscles. Nat Med. 2014;20:255-64 pubmed publisher
Shi Z, Chen J, Li C, An N, Wang Z, Yang S, et al. Antitumor effects of concanavalin A and Sophora flavescens lectin in vitro and in vivo. Acta Pharmacol Sin. 2014;35:248-56 pubmed publisher
Li Q, Yu P, Wang W, Zhang P, Yang H, Li S, et al. Gene expression profiles of various cytokines in mesenchymal stem cells derived from umbilical cord tissue and bone marrow following infection with human cytomegalovirus. Cell Mol Biol Lett. 2014;19:140-57 pubmed publisher
Banerjee S, Lu J, Cai Q, Sun Z, Jha H, Robertson E. EBNA3C augments Pim-1 mediated phosphorylation and degradation of p21 to promote B-cell proliferation. PLoS Pathog. 2014;10:e1004304 pubmed publisher
Stead M, Wright S. Nac1 interacts with the POZ-domain transcription factor, Miz1. Biosci Rep. 2014;34: pubmed publisher
Li W, Zheng J, Deng J, You Y, Wu H, Li N, et al. Increased levels of the long intergenic non-protein coding RNA POU3F3 promote DNA methylation in esophageal squamous cell carcinoma cells. Gastroenterology. 2014;146:1714-26.e5 pubmed publisher
Douthwright S, Sluder G. Link between DNA damage and centriole disengagement/reduplication in untransformed human cells. J Cell Physiol. 2014;229:1427-36 pubmed publisher
Li H, Qi Y, Li C, Braseth L, Gao Y, Shabashvili A, et al. Angiotensin type 2 receptor-mediated apoptosis of human prostate cancer cells. Mol Cancer Ther. 2009;8:3255-65 pubmed publisher
Hu J, Chen Z, Xia D, Wu J, Xu H, Ye Z. Promoter-associated small double-stranded RNA interacts with heterogeneous nuclear ribonucleoprotein A2/B1 to induce transcriptional activation. Biochem J. 2012;447:407-16 pubmed publisher
Baghy K, Horvath Z, Regős E, Kiss K, Schaff Z, Iozzo R, et al. Decorin interferes with platelet-derived growth factor receptor signaling in experimental hepatocarcinogenesis. FEBS J. 2013;280:2150-64 pubmed publisher
Stiles J, Amaya C, Rains S, Diaz D, Pham R, Battiste J, et al. Targeting of beta adrenergic receptors results in therapeutic efficacy against models of hemangioendothelioma and angiosarcoma. PLoS ONE. 2013;8:e60021 pubmed publisher
Ji Y, Xie M, Lan H, Zhang Y, Long Y, Weng H, et al. PRR11 is a novel gene implicated in cell cycle progression and lung cancer. Int J Biochem Cell Biol. 2013;45:645-56 pubmed publisher
Cheng X, Xia W, Yang J, Hsu J, Lang J, Chou C, et al. Activation of murine double minute 2 by Akt in mammary epithelium delays mammary involution and accelerates mammary tumorigenesis. Cancer Res. 2010;70:7684-9 pubmed publisher
Findeisen H, Gizard F, Zhao Y, Qing H, Heywood E, Jones K, et al. Epigenetic regulation of vascular smooth muscle cell proliferation and neointima formation by histone deacetylase inhibition. Arterioscler Thromb Vasc Biol. 2011;31:851-60 pubmed publisher
Thorne J, Maguire O, Doig C, Battaglia S, Fehr L, Sucheston L, et al. Epigenetic control of a VDR-governed feed-forward loop that regulates p21(waf1/cip1) expression and function in non-malignant prostate cells. Nucleic Acids Res. 2011;39:2045-56 pubmed publisher
Uetake Y, Sluder G. Prolonged prometaphase blocks daughter cell proliferation despite normal completion of mitosis. Curr Biol. 2010;20:1666-71 pubmed publisher
Hwang-Verslues W, Sladek F. Nuclear receptor hepatocyte nuclear factor 4alpha1 competes with oncoprotein c-Myc for control of the p21/WAF1 promoter. Mol Endocrinol. 2008;22:78-90 pubmed
Romito A, Lonardo E, Roma G, Minchiotti G, Ballabio A, Cobellis G. Lack of sik1 in mouse embryonic stem cells impairs cardiomyogenesis by down-regulating the cyclin-dependent kinase inhibitor p57kip2. PLoS ONE. 2010;5:e9029 pubmed publisher
Kim J, Esteve P, Jacobsen S, Pradhan S. UHRF1 binds G9a and participates in p21 transcriptional regulation in mammalian cells. Nucleic Acids Res. 2009;37:493-505 pubmed publisher
Seluanov A, Hine C, Bozzella M, Hall A, Sasahara T, Ribeiro A, et al. Distinct tumor suppressor mechanisms evolve in rodent species that differ in size and lifespan. Aging Cell. 2008;7:813-23 pubmed publisher
Gizard F, Zhao Y, Findeisen H, Qing H, Cohn D, Heywood E, et al. Transcriptional regulation of S phase kinase-associated protein 2 by NR4A orphan nuclear receptor NOR1 in vascular smooth muscle cells. J Biol Chem. 2011;286:35485-93 pubmed publisher
Spurlock C, Tossberg J, Fuchs H, Olsen N, Aune T. Methotrexate increases expression of cell cycle checkpoint genes via JNK activation. Arthritis Rheum. 2012;64:1780-9 pubmed publisher
Cotto-Rios X, Jones M, Busino L, Pagano M, Huang T. APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage. J Cell Biol. 2011;194:177-86 pubmed publisher
Findeisen H, Pearson K, Gizard F, Zhao Y, Qing H, Jones K, et al. Oxidative stress accumulates in adipose tissue during aging and inhibits adipogenesis. PLoS ONE. 2011;6:e18532 pubmed publisher
Pont A, Sadri N, Hsiao S, Smith S, Schneider R. mRNA decay factor AUF1 maintains normal aging, telomere maintenance, and suppression of senescence by activation of telomerase transcription. Mol Cell. 2012;47:5-15 pubmed publisher
Truong A, Kretz M, Ridky T, Kimmel R, Khavari P. p63 regulates proliferation and differentiation of developmentally mature keratinocytes. Genes Dev. 2006;20:3185-97 pubmed
Gu B, Bessler M, Mason P. A pathogenic dyskerin mutation impairs proliferation and activates a DNA damage response independent of telomere length in mice. Proc Natl Acad Sci U S A. 2008;105:10173-8 pubmed publisher
Shirakami Y, Gottesman M, Blaner W. Diethylnitrosamine-induced hepatocarcinogenesis is suppressed in lecithin:retinol acyltransferase-deficient mice primarily through retinoid actions immediately after carcinogen administration. Carcinogenesis. 2012;33:268-74 pubmed publisher
Thomas S, Malzer E, Ordonez A, Dalton L, van 't Wout E, Liniker E, et al. p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress. J Biol Chem. 2013;288:7606-17 pubmed publisher
Yang Y, Ikezoe T, Nishioka C, Bandobashi K, Takeuchi T, Adachi Y, et al. NFV, an HIV-1 protease inhibitor, induces growth arrest, reduced Akt signalling, apoptosis and docetaxel sensitisation in NSCLC cell lines. Br J Cancer. 2006;95:1653-62 pubmed
García-Serrano L, Gomez-Ferreria M, Contreras-Jurado C, Segrelles C, Paramio J, Aranda A. The thyroid hormone receptors modulate the skin response to retinoids. PLoS ONE. 2011;6:e23825 pubmed publisher
Arzenani M, Zade A, Ming Y, Vijverberg S, Zhang Z, Khan Z, et al. Genomic DNA hypomethylation by histone deacetylase inhibition implicates DNMT1 nuclear dynamics. Mol Cell Biol. 2011;31:4119-28 pubmed publisher
Minelli R, Serpe L, Pettazzoni P, Minero V, Barrera G, Gigliotti C, et al. Cholesteryl butyrate solid lipid nanoparticles inhibit the adhesion and migration of colon cancer cells. Br J Pharmacol. 2012;166:587-601 pubmed publisher
Boheler K, Joodi R, Qiao H, Juhasz O, Urick A, Chuppa S, et al. Embryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration. Stem Cells Int. 2011;2011:214203 pubmed
Xie X, Wei M, Kakehashi A, Yamano S, Tajiri M, Wanibuchi H. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) promotes mouse hepatocarcinogenesis by activating transforming growth factor-β and Wnt/β-catenin signaling pathways. Toxicol Sci. 2012;125:392-400 pubmed publisher
Chang P, Chi C, Chau G, Li F, Tsai Y, Wu J, et al. DDX3, a DEAD box RNA helicase, is deregulated in hepatitis virus-associated hepatocellular carcinoma and is involved in cell growth control. Oncogene. 2006;25:1991-2003 pubmed
Zou S, Ai K, Wang Z, Yuan Z, Yan J, Zheng Q. The role of Med19 in the proliferation and tumorigenesis of human hepatocellular carcinoma cells. Acta Pharmacol Sin. 2011;32:354-60 pubmed publisher
Lam M, Carmichael A, Griffiths H. An aqueous extract of Fagonia cretica induces DNA damage, cell cycle arrest and apoptosis in breast cancer cells via FOXO3a and p53 expression. PLoS ONE. 2012;7:e40152 pubmed publisher
Mendias C, Bakhurin K, Faulkner J. Tendons of myostatin-deficient mice are small, brittle, and hypocellular. Proc Natl Acad Sci U S A. 2008;105:388-93 pubmed
Sigl R, Wandke C, Rauch V, Kirk J, Hunt T, Geley S. Loss of the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis. J Cell Sci. 2009;122:4208-17 pubmed publisher
Manceau M, Gros J, Savage K, Thomé V, McPherron A, PATERSON B, et al. Myostatin promotes the terminal differentiation of embryonic muscle progenitors. Genes Dev. 2008;22:668-81 pubmed publisher
Polioudakis D, Bhinge A, Killion P, Lee B, Abell N, Iyer V. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res. 2013;41:2239-54 pubmed publisher
Chen L, Wei T, Si X, Wang Q, Li Y, Leng Y, et al. Lysine acetyltransferase GCN5 potentiates the growth of non-small cell lung cancer via promotion of E2F1, cyclin D1, and cyclin E1 expression. J Biol Chem. 2013;288:14510-21 pubmed publisher
Liu F, Lai M, Chen Y, Lin W, Chang S, Sheu M, et al. Elucidating the inhibitory mechanisms of the ethanolic extract of the fruiting body of the mushroom Antrodia cinnamomea on the proliferation and migration of murine leukemia WEHI-3 cells and their tumorigenicity in a BALB/c allograft tumor model. Phytomedicine. 2013;20:874-82 pubmed publisher
Pettazzoni P, Pizzimenti S, Toaldo C, Sotomayor P, Tagliavacca L, Liu S, et al. Induction of cell cycle arrest and DNA damage by the HDAC inhibitor panobinostat (LBH589) and the lipid peroxidation end product 4-hydroxynonenal in prostate cancer cells. Free Radic Biol Med. 2011;50:313-22 pubmed publisher
product information
Catalog Number :
ab7960
Product Name :
p21 antibody
Host Species :
Rabbit
Clonality :
polyclonal
Product Type :
Primary antibodies
Size :
1 ml
SwissProtID :
P38936
Isotype :
IgG
CrossReactivity :
Reacts with: Human, Mouse, Rat.
Application Summary :
Flow Cyt, ICC, ICC/IF, IHC-Fr, IP, WB
company information
Abcam
330 Cambridge Science Park
Cambridge
CB4 0FL UK
orders@abcam.com
44 (0)1223 696000

1 Kendall Square, Ste 341
Cambridge, MA 02139-1517
USA
us.orders@abcam.com
617 225 2272, 888-77-ABCAM (22226)

www.abcam.com
888-7722226
headquarters: UK