This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-O-Linked N-Acetylglucosamine antibody [RL2]
catalog :
ab2739
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
RL2
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, dot blot, immunohistochemistry knockout validation
citations: 55
Reference
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Pellegrini F, De Martino S, Fianco G, Ventura I, Valente D, Fiore M, et al. Blockage of autophagosome-lysosome fusion through SNAP29 O-GlcNAcylation promotes apoptosis via ROS production. Autophagy. 2023;19:2078-2093 pubmed publisher
Cioce A, Calle B, Rizou T, Lowery S, Bridgeman V, Mahoney K, et al. Cell-specific bioorthogonal tagging of glycoproteins. Nat Commun. 2022;13:6237 pubmed publisher
Mathew M, Abramowitz L, Donaldson J, Hanover J. Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3. J Biol Chem. 2022;298:101743 pubmed publisher
Massman L, Pereckas M, Zwagerman N, Olivier Van Stichelen S. O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells. Endocrinology. 2021;162: pubmed publisher
Gonzalez Rellan M, Fondevila M, Fernandez U, Rodríguez A, Varela Rey M, Veyrat Durebex C, et al. O-GlcNAcylated p53 in the liver modulates hepatic glucose production. Nat Commun. 2021;12:5068 pubmed publisher
Basu H, Pekkurnaz G, Falk J, Wei W, Chin M, Steen J, et al. FHL2 anchors mitochondria to actin and adapts mitochondrial dynamics to glucose supply. J Cell Biol. 2021;220: pubmed publisher
Liu X, Chen X, Xiao M, Zhu Y, Gong R, Liu J, et al. RANBP2 Activates O-GlcNAcylation through Inducing CEBPα-Dependent OGA Downregulation to Promote Hepatocellular Carcinoma Malignant Phenotypes. Cancers (Basel). 2021;13: pubmed publisher
Yoo T, Mitchison T. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol. 2021;220: pubmed publisher
Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics. 2021;11:5650-5674 pubmed publisher
Hu J, Gao Q, Yang Y, Xia J, Zhang W, Chen Y, et al. Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation. Theranostics. 2021;11:805-823 pubmed publisher
Liu B, Salgado O, Singh S, Hippen K, Maynard J, Burlingame A, et al. The lineage stability and suppressive program of regulatory T cells require protein O-GlcNAcylation. Nat Commun. 2019;10:354 pubmed publisher
Hrit J, Goodrich L, Li C, Wang B, Nie J, Cui X, et al. OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development. elife. 2018;7: pubmed publisher
Krause M, Love D, Ghosh S, Wang P, Yun S, Fukushige T, et al. Nutrient-Driven O-GlcNAcylation at Promoters Impacts Genome-Wide RNA Pol II Distribution. Front Endocrinol (Lausanne). 2018;9:521 pubmed publisher
Zhao M, Xiong X, Ren K, Xu B, Cheng M, Sahu C, et al. Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation. EMBO Mol Med. 2018;10: pubmed publisher
Sekine H, Okazaki K, Kato K, Alam M, Shima H, Katsuoka F, et al. O-GlcNAcylation Signal Mediates Proteasome Inhibitor Resistance in Cancer Cells by Stabilizing NRF1. Mol Cell Biol. 2018;38: pubmed publisher
Gu Y, Yang Y, Wan B, Li M, Guo L. Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity. Biochem Pharmacol. 2018;152:21-33 pubmed publisher
Dodson M, Liu P, Jiang T, Ambrose A, Luo G, Rojo de la Vega M, et al. Increased O-GlcNAcylation of SNAP29 Drives Arsenic-Induced Autophagic Dysfunction. Mol Cell Biol. 2018;38: pubmed publisher
Ruan H, Ma Y, Torres S, Zhang B, Feriod C, Heck R, et al. Calcium-dependent O-GlcNAc signaling drives liver autophagy in adaptation to starvation. Genes Dev. 2017;31:1655-1665 pubmed publisher
Zhu Y, Zhao S, Deng Y, Gordillo R, Ghaben A, Shao M, et al. Hepatic GALE Regulates Whole-Body Glucose Homeostasis by Modulating Tff3 Expression. Diabetes. 2017;66:2789-2799 pubmed publisher
Qin W, Lv P, Fan X, Quan B, Zhu Y, Qin K, et al. Quantitative time-resolved chemoproteomics reveals that stable O-GlcNAc regulates box C/D snoRNP biogenesis. Proc Natl Acad Sci U S A. 2017;114:E6749-E6758 pubmed publisher
Tan E, McGreal S, Graw S, Tessman R, Koppel S, Dhakal P, et al. Sustained O-GlcNAcylation reprograms mitochondrial function to regulate energy metabolism. J Biol Chem. 2017;292:14940-14962 pubmed publisher
Ida S, Morino K, Sekine O, Ohashi N, Kume S, Chano T, et al. Diverse metabolic effects of O-GlcNAcylation in the pancreas but limited effects in insulin-sensitive organs in mice. Diabetologia. 2017;60:1761-1769 pubmed publisher
Magescas J, Sengmanivong L, Viau A, Mayeux A, Dang T, Burtin M, et al. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Sci Rep. 2017;7:1474 pubmed publisher
Guo H, Zhang B, Nairn A, Nagy T, Moremen K, Buckhaults P, et al. O-Linked N-Acetylglucosamine (O-GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1. J Biol Chem. 2017;292:4123-4137 pubmed publisher
Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
Jo Y, Park N, Park S, Kim B, Shin J, Jo D, et al. O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity. Oncotarget. 2016;7:57186-57196 pubmed publisher
Swamy M, Pathak S, Grzes K, Damerow S, Sinclair L, van Aalten D, et al. Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy. Nat Immunol. 2016;17:712-20 pubmed publisher
Shi J, Sharif S, Ruijtenbeek R, Pieters R. Activity Based High-Throughput Screening for Novel O-GlcNAc Transferase Substrates Using a Dynamic Peptide Microarray. PLoS ONE. 2016;11:e0151085 pubmed publisher
Kellner N, Schwarz J, Sturm M, Fernandez Martinez J, Griesel S, Zhang W, et al. Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies. Sci Rep. 2016;6:20937 pubmed publisher
Gomes L, Odedra D, Dikic I, Pohl C. Autophagy and modular restructuring of metabolism control germline tumor differentiation and proliferation in C. elegans. Autophagy. 2016;12:529-46 pubmed publisher
Ding X, Jiang W, Zhou P, Liu L, Wan X, Yuan X, et al. Mixed Lineage Leukemia 5 (MLL5) Protein Stability Is Cooperatively Regulated by O-GlcNac Transferase (OGT) and Ubiquitin Specific Protease 7 (USP7). PLoS ONE. 2015;10:e0145023 pubmed publisher
Ledee D, Smith L, Bruce M, Kajimoto M, Isern N, Portman M, et al. c-Myc Alters Substrate Utilization and O-GlcNAc Protein Posttranslational Modifications without Altering Cardiac Function during Early Aortic Constriction. PLoS ONE. 2015;10:e0135262 pubmed publisher
Gagnon J, Daou S, Zamorano N, Iannantuono N, Hammond Martel I, Mashtalir N, et al. Undetectable histone O-GlcNAcylation in mammalian cells. Epigenetics. 2015;10:677-91 pubmed publisher
Ledee D, Kajimoto M, O Kelly Priddy C, Olson A, Isern N, Robillard Frayne I, et al. Pyruvate modifies metabolic flux and nutrient sensing during extracorporeal membrane oxygenation in an immature swine model. Am J Physiol Heart Circ Physiol. 2015;309:H137-46 pubmed publisher
Zhang Z, Tan E, VandenHull N, Peterson K, Slawson C. O-GlcNAcase Expression is Sensitive to Changes in O-GlcNAc Homeostasis. Front Endocrinol (Lausanne). 2014;5:206 pubmed publisher
Ganguly R, Sahu S, Chavez R, Raman P. Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro. Am J Physiol Cell Physiol. 2015;308:C111-22 pubmed publisher
Myslicki J, Shearer J, Hittel D, Hughey C, Belke D. O-GlcNAc modification is associated with insulin sensitivity in the whole blood of healthy young adult males. Diabetol Metab Syndr. 2014;6:96 pubmed publisher
Liu X, Li L, Wang Y, Yan H, Ma X, Wang P, et al. A peptide panel investigation reveals the acceptor specificity of O-GlcNAc transferase. FASEB J. 2014;28:3362-72 pubmed publisher
Tashima Y, Stanley P. Antibodies that detect O-linked ?-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem. 2014;289:11132-42 pubmed publisher
Medford H, Cox E, Miller L, Marsh S. Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts. Am J Physiol Regul Integr Comp Physiol. 2014;306:R519-26 pubmed publisher
Mellone M, Kestoras D, Andrews M, Dassie E, Crowther R, Stokin G, et al. Tau pathology is present in vivo and develops in vitro in sensory neurons from human P301S tau transgenic mice: a system for screening drugs against tauopathies. J Neurosci. 2013;33:18175-89 pubmed publisher
Feng Z, Hui Y, Ling L, Xiaoyan L, Yuqiu W, Peng W, et al. FBXW10 is negatively regulated in transcription and expression level by protein O-GlcNAcylation. Biochem Biophys Res Commun. 2013;438:427-32 pubmed publisher
Medford H, Porter K, Marsh S. Immediate effects of a single exercise bout on protein O-GlcNAcylation and chromatin regulation of cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2013;305:H114-23 pubmed publisher
Li M, Ruan H, Hughes M, Lee J, Singh J, Jones S, et al. O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell Metab. 2013;17:303-10 pubmed publisher
Deplus R, Delatte B, Schwinn M, Defrance M, Méndez J, Murphy N, et al. TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS. EMBO J. 2013;32:645-55 pubmed publisher
Yi T, Cheema Y, Tremble S, Bell S, Chen Z, Subramanian M, et al. Zinc-induced cardiomyocyte relaxation in a rat model of hyperglycemia is independent of myosin isoform. Cardiovasc Diabetol. 2012;11:135 pubmed publisher
Fong J, Nguyen B, Bridger R, Medrano E, Wells L, Pan S, et al. ?-N-Acetylglucosamine (O-GlcNAc) is a novel regulator of mitosis-specific phosphorylations on histone H3. J Biol Chem. 2012;287:12195-203 pubmed publisher
Pfister F, Riedl E, Wang Q, Vom Hagen F, Deinzer M, Harmsen M, et al. Oral carnosine supplementation prevents vascular damage in experimental diabetic retinopathy. Cell Physiol Biochem. 2011;28:125-36 pubmed publisher
Riedl E, Pfister F, Braunagel M, Brinkkötter P, Sternik P, Deinzer M, et al. Carnosine prevents apoptosis of glomerular cells and podocyte loss in STZ diabetic rats. Cell Physiol Biochem. 2011;28:279-88 pubmed publisher
Love D, Ghosh S, Mondoux M, Fukushige T, Wang P, Wilson M, et al. Dynamic O-GlcNAc cycling at promoters of Caenorhabditis elegans genes regulating longevity, stress, and immunity. Proc Natl Acad Sci U S A. 2010;107:7413-8 pubmed publisher
Pantaleon M, Tan H, Kafer G, Kaye P. Toxic effects of hyperglycemia are mediated by the hexosamine signaling pathway and o-linked glycosylation in early mouse embryos. Biol Reprod. 2010;82:751-8 pubmed publisher
Xue M, Qian Q, Adaikalakoteswari A, Rabbani N, Babaei Jadidi R, Thornalley P. Activation of NF-E2-related factor-2 reverses biochemical dysfunction of endothelial cells induced by hyperglycemia linked to vascular disease. Diabetes. 2008;57:2809-17 pubmed publisher
Ahrend M, Käberich A, Fergen M, Schmitz B. Immunochemical methods for the rapid screening of the o-glycosidically linked N-acetylglucosamine modification of proteins. Methods Mol Biol. 2008;446:267-80 pubmed publisher
Golks A, Tran T, Goetschy J, Guerini D. Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation. EMBO J. 2007;26:4368-79 pubmed
product information
CatalogNumber :
ab2739
ItemName :
Anti-O-Linked N-Acetylglucosamine antibody [RL2]
Category :
Antibody
Host :
Mouse
Clonality :
Monoclonal
Conjugate :
Unconjugated
Clone Name :
RL2
Size :
100 µg
Application Summary :
ChIP/Chip, Dot, ICC/IF, IHC-Fr, IP, WB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
Stefan.Pritchard@abcam.com, eCommerce@abcam.com, Vicky.Yang@abcam.com
https://www.abcam.com
888-7722226
headquarters: UK