This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-HIF-1 alpha antibody - ChIP Grade
catalog :
ab2185
quantity :
100 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:2000; loading ...; fig s7g
Torretta S, Scagliola A, Ricci L, Mainini F, Di Marco S, Cuccovillo I, et al. D-mannose suppresses macrophage IL-1β production. Nat Commun. 2020;11:6343 pubmed publisher
  • western blot; mouse; loading ...; fig 1a, 5b
Lee Y, Riopel M, Cabrales P, Bandyopadhyay G. Hepatocyte-specific HIF-1α ablation improves obesity-induced glucose intolerance by reducing first-pass GLP-1 degradation. Sci Adv. 2019;5:eaaw4176 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5b
  • western blot; mouse; loading ...; fig 5d
Liu F, Fan D, Yang Z, Tang N, Guo Z, Ma S, et al. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis. Cell Death Dis. 2019;10:480 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 6a
  • immunoprecipitation; human; loading ...; fig 4e
  • western blot; human; loading ...; fig 1d
Jia Y, Li H, Wang J, Wang Y, Zhang P, Ma N, et al. Phosphorylation of 14-3-3ζ links YAP transcriptional activation to hypoxic glycolysis for tumorigenesis. Oncogenesis. 2019;8:31 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 4b
Lin P, Wu M, Qin J, Yang J, Ye C, Wang C. Magnesium lithospermate B improves renal hemodynamics and reduces renal oxygen consumption in 5/6th renal ablation/infarction rats. BMC Nephrol. 2019;20:49 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:8000; fig 9c
Juhasz A, Markel S, Gaur S, Liu H, Lu J, Jiang G, et al. NADPH oxidase 1 supports proliferation of colon cancer cells by modulating reactive oxygen species-dependent signal transduction. J Biol Chem. 2017;292:7866-7887 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 8a
Lajko M, Cardona H, Taylor J, Shah R, Farrow K, Fawzi A. Hyperoxia-Induced Proliferative Retinopathy: Early Interruption of Retinal Vascular Development with Severe and Irreversible Neurovascular Disruption. PLoS ONE. 2016;11:e0166886 pubmed publisher
  • western blot; human; loading ...; fig s7
Kwak J, Lee N, Lee H, Hong I, Nam J. HIF2?/EFEMP1 cascade mediates hypoxic effects on breast cancer stem cell hierarchy. Oncotarget. 2016;7:43518-43533 pubmed publisher
  • chromatin immunoprecipitation; human; 1:150; loading ...; fig 3b
  • immunocytochemistry; human; 1:500; loading ...; fig 6b
Brooks D, Schwab L, Krutilina R, Parke D, Sethuraman A, Hoogewijs D, et al. ITGA6 is directly regulated by hypoxia-inducible factors and enriches for cancer stem cell activity and invasion in metastatic breast cancer models. Mol Cancer. 2016;15:26 pubmed publisher
  • ChIP-Seq; human; fig 2
de Bruin A, A Cornelissen P, Kirchmaier B, Mokry M, Iich E, Nirmala E, et al. Genome-wide analysis reveals NRP1 as a direct HIF1?-E2F7 target in the regulation of motorneuron guidance in vivo. Nucleic Acids Res. 2016;44:3549-66 pubmed publisher
  • western blot; mouse; fig 6
David V, Martin A, Isakova T, Spaulding C, Qi L, Ramirez V, et al. Inflammation and functional iron deficiency regulate fibroblast growth factor 23 production. Kidney Int. 2016;89:135-46 pubmed publisher
Teixeira V, Mohamed I, Lavoie J. Neonatal Vitamin C and Cysteine Deficiencies Program Adult Hepatic Glutathione and Specific Activities of Glucokinase, Phosphofructokinase, and Acetyl-CoA Carboxylase in Guinea Pigs' Livers. Antioxidants (Basel). 2021;10: pubmed publisher
Pan W, Song X, Hu Q, Zhang Y. miR-485 inhibits histone deacetylase HDAC5, HIF1α and PFKFB3 expression to alleviate epilepsy in cellular and rodent models. Aging (Albany NY). 2021;13:14416-14432 pubmed publisher
Wu H, Liu X, Gao Z, Lin M, Zhao X, Sun Y, et al. Icaritin Provides Neuroprotection in Parkinson's Disease by Attenuating Neuroinflammation, Oxidative Stress, and Energy Deficiency. Antioxidants (Basel). 2021;10: pubmed publisher
Zilli F, Marques Ramos P, Auf der Maur P, Jehanno C, Sethi A, Coissieux M, et al. The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells. EMBO Mol Med. 2021;13:e13162 pubmed publisher
Tran M, Bibby B, Yang L, Lo F, Warren A, Shukla D, et al. Independence of HIF1a and androgen signaling pathways in prostate cancer. BMC Cancer. 2020;20:469 pubmed publisher
Hodrea J, Balogh D, Hosszú A, Lenart L, Besztercei B, Koszegi S, et al. Reduced O-GlcNAcylation and tubular hypoxia contribute to the antifibrotic effect of SGLT2 inhibitor dapagliflozin in the diabetic kidney. Am J Physiol Renal Physiol. 2020;318:F1017-F1029 pubmed publisher
Pak J, Yi J, Ryu S, Kim I, Kim J, Baek H, et al. Induction of Redox-Active Gene Expression by CoCl2 Ameliorates Oxidative Stress-Mediated Injury of Murine Auditory Cells. Antioxidants (Basel). 2019;8: pubmed publisher
Boso D, Rampazzo E, Zanon C, Bresolin S, Maule F, Porcù E, et al. HIF-1α/Wnt signaling-dependent control of gene transcription regulates neuronal differentiation of glioblastoma stem cells. Theranostics. 2019;9:4860-4877 pubmed publisher
Qin H, Yu H, Sheng J, Zhang D, Shen N, Liu L, et al. PI3Kgamma Inhibitor Attenuates Immunosuppressive Effect of Poly(l-Glutamic Acid)-Combretastatin A4 Conjugate in Metastatic Breast Cancer. Adv Sci (Weinh). 2019;6:1900327 pubmed publisher
Silberman A, Goldman O, Boukobza Assayag O, Jacob A, Rabinovich S, Adler L, et al. Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer. Cancer Res. 2019;79:518-533 pubmed publisher
Cortés E, Lachowski D, Robinson B, Sarper M, Teppo J, Thorpe S, et al. Tamoxifen mechanically reprograms the tumor microenvironment via HIF-1A and reduces cancer cell survival. EMBO Rep. 2019;20: pubmed publisher
Wang Y, Ren K, Xie L, Sun W, Liu Y, Li S. Effect of Repeated Injection of Iodixanol on Renal Function in Healthy Wistar Rats Using Functional MRI. Biomed Res Int. 2018;2018:7272485 pubmed publisher
Shi Q, Liu X, Wang N, Zheng X, Fu J, Zheng J. Nitric oxide from brain microvascular endothelial cells may initiate the compensatory response to mild hypoxia of astrocytes in a hypoxia-inducible factor-1? dependent manner. Am J Transl Res. 2016;8:4735-4749 pubmed
Bayin N, Frenster J, Kane J, Rubenstein J, Modrek A, Baitalmal R, et al. GPR133 (ADGRD1), an adhesion G-protein-coupled receptor, is necessary for glioblastoma growth. Oncogenesis. 2016;5:e263 pubmed publisher
Thuringer D, Boucher J, Jego G, Pernet N, Cronier L, Hammann A, et al. Transfer of functional microRNAs between glioblastoma and microvascular endothelial cells through gap junctions. Oncotarget. 2016;7:73925-73934 pubmed publisher
Li C, Liu J, Yu B, Liu R, Dong C, Li S. Notch signaling represses hypoxia-inducible factor-1?-induced activation of Wnt/?-catenin signaling in osteoblasts under cobalt-mimicked hypoxia. Mol Med Rep. 2016;14:689-96 pubmed publisher
Suyama K, Silagi E, Choi H, Sakabe K, Mochida J, Shapiro I, et al. Circadian factors BMAL1 and ROR? control HIF-1? transcriptional activity in nucleus pulposus cells: implications in maintenance of intervertebral disc health. Oncotarget. 2016;7:23056-71 pubmed publisher
Wollenick K, Hu J, Kristiansen G, Schraml P, Rehrauer H, Berchner Pfannschmidt U, et al. Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Nucleic Acids Res. 2012;40:1928-43 pubmed publisher
Stiehl D, Bordoli M, Abreu Rodríguez I, Wollenick K, Schraml P, Gradin K, et al. Non-canonical HIF-2? function drives autonomous breast cancer cell growth via an AREG-EGFR/ErbB4 autocrine loop. Oncogene. 2012;31:2283-97 pubmed publisher
product information
CatalogNumber :
ab2185
ItemName :
Anti-HIF-1 alpha antibody - ChIP Grade
Product Type :
Primary Antibodies
Host Species :
Rabbit
Clonality :
Polyclonal
Conjugate :
Not Conjugated
Antigen Modification :
No Modifications
Size :
100 µl
SpeciesSummary :
Gpig, Hu, Mk, Ms, Rat, Xl
Uses :
ChIP, GSA, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
https://www.abcam.com
888-7722226
headquarters: UK